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PMID: 6096689 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The minimum amount of homology required for homologous recombination in mammalian cells.

Molecular and cellular biology ·Vol. 4 ·No. 11 ·1984-11-00 ·Pages 2253-8

Rubnitz J, Subramani S

Abstract

Although DNA sequence homology is believed to be a prerequisite for homologous recombination events in procaryotes and eucaryotes, no systematic study has been done on the minimum amount of homology required for homologous recombination in mammalian cells. We have used simian virus 40-pBR322 hybrid plasmids constructed in vitro as substrates to quantitate intramolecular homologous recombination in cultured monkey cells. Excision of wild-type simian virus 40 DNA by homologous recombination was scored by the viral plaque assay. Using a series of plasmids containing 0 to 243 base pairs of homology, we have shown that the recombination frequency decreases as the homology is reduced, with the sharpest drop in recombination frequency occurring when the homology was reduced from 214 to 163 base pairs. However, low recombination frequencies were also observed with as little as 14 base pairs of homology.

MeSH Terms
Animals Base Sequence Cells, Cultured Coliphages/genetics DNA/genetics Haplorhini Plasmids Recombination, Genetic Simian virus 40/genetics
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rubnitz J
Subramani S
References (41)
41 references, click to expand
  1. Evidence for intrachromosomal gene conversion in cultured mouse cells.
    Cell. 1983 Nov;35(1):157-65 PMID: 6578876
  2. Role of short regions of homology in intermolecular illegitimate recombination events.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2452-6 PMID: 6302684
  3. By searching processively RecA protein pairs DNA molecules that share a limited stretch of homology.
    Cell. 1983 Sep;34(2):647-54 PMID: 6616624
  4. Defective simian virus 40 genomes: isolation and growth of individual clones.
    Virology. 1974 Nov;62(1):112-24 PMID: 4370797
  5. Identification and nucleotide sequence of a diversity DNA segment (D) of immunoglobulin heavy-chain genes.
    Nature. 1981 Apr 16;290(5807):562-5 PMID: 6783961
  6. How damaged is the biologically active subpopulation of transfected DNA?
    Mol Cell Biol. 1984 Mar;4(3):387-98 PMID: 6325874
  7. Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4827-31 PMID: 6576360
  8. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  9. Recombination between temperature-sensitive mutants of simian virus 40.
    Virology. 1974 Jan;57(1):161-74 PMID: 4362021
  10. Localization of sister chromatid exchanges in human chromosomes.
    Science. 1974 Jul 5;185(4145):74-6 PMID: 4134970
  11. Sister Chromatid Exchanges in Tritium-Labeled Chromosomes.
    Genetics. 1958 May;43(3):515-29 PMID: 17247775
  12. Somatic cells efficiently join unrelated DNA segments end-to-end.
    Mol Cell Biol. 1982 Oct;2(10):1258-69 PMID: 6294502
  13. Recombination of DNA molecules.
    Prog Nucleic Acid Res Mol Biol. 1966;5:315-37 PMID: 5337698
  14. The FLP protein of the yeast 2-microns plasmid: expression of a eukaryotic genetic recombination system in Escherichia coli.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4223-7 PMID: 6308608
  15. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  16. Determination of the amount of homology required for recombination in bacteriophage T4.
    Cell. 1982 Nov;31(1):25-33 PMID: 6297750
  17. Analysis of recombination in mammalian cells using SV40 genome segments having homologous overlapping termini.
    Nucleic Acids Res. 1980 Jun 25;8(12):2725-36 PMID: 6253890
  18. Recombination between endogenous and exogenous simian virus 40 genes. III. Rescue of SV40 tsA and tsBC mutants by passage in permissive transformed monkey lines.
    Virology. 1980 Jul 15;104(1):73-83 PMID: 6249030
  19. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  20. Extrachromosomal circular copies of an 'inter-Alu' unstable sequence in human DNA are amplified during in vitro and in vivo ageing.
    Nature. 1983 Feb 3;301(5899):394-8 PMID: 6823315
  21. Analysis of the nucleotide sequence of an invertible controlling element.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4196-200 PMID: 6933466
  22. Transposon-mediated site-specific recombination: identification of three binding sites for resolvase at the res sites of gamma delta and Tn3.
    Cell. 1982 Aug;30(1):19-27 PMID: 6290077
  23. Genome instability in a region of human DNA enriched in Alu repeat sequences.
    Nature. 1982 Mar 18;296(5854):219-25 PMID: 6278320
  24. The kinetics of adenovirus recombination in homotypic and heterotypic genetic crosses.
    Virology. 1980 Mar;101(2):503-15 PMID: 7361457
  25. DNA inversions in the chromosome of Escherichia coli and in bacteriophage Mu: relationship to other site-specific recombination systems.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5355-8 PMID: 6310572
  26. Recombination during gene transfer into mouse cells can restore the function of deleted genes.
    Science. 1983 Jan 14;219(4581):174-6 PMID: 6294829
  27. A complete immunoglobulin gene is created by somatic recombination.
    Cell. 1978 Sep;15(1):1-14 PMID: 100225
  28. P1 site-specific recombination: nucleotide sequence of the recombining sites.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3398-402 PMID: 6954485
  29. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  30. Molecular aspects of genetic exchange and gene conversion.
    Genetics. 1974 Sep;78(1):273-87 PMID: 4613609
  31. Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2002-6 PMID: 6132383
  32. Homologous and nonhomologous recombination in monkey cells.
    Mol Cell Biol. 1983 Jun;3(6):1040-52 PMID: 6308421
  33. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.
    Cell. 1982 Jun;29(2):319-28 PMID: 6288254
  34. Viral integration and excision: structure of the lambda att sites.
    Science. 1977 Sep 16;197(4309):1147-60 PMID: 331474
  35. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  36. Inversions of specific DNA segments in flagellar phase variation of Salmonella and inversion systems of bacteriophages P1 and Mu.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7338-41 PMID: 7012837
  37. Homologous pairing and strand exchange in genetic recombination.
    Annu Rev Genet. 1982;16:405-37 PMID: 6297377
  38. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  39. Resolution of cointegrates between transposons gamma delta and Tn3 defines the recombination site.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3428-32 PMID: 6267590
  40. G inversion in bacteriophage Mu: a novel way of gene splicing.
    Nature. 1982 May 27;297(5864):339-42 PMID: 6210848
  41. Simian virus 40 recombinants are produced at high frequency during infection with genetically mixed oligomeric DNA.
    Proc Natl Acad Sci U S A. 1979 Jun;76(6):2876-80 PMID: 223162
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.11.2253-2258.1984
Published
1984-11-00
Pages
2253-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369052
Subset
IM
Grants
NIGMS NIH HHS · GM31253-02 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.11.2253-2258.1984 PMC Full Text View journal details
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