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PMID: 9852143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A novel nuclear import pathway for the transcription factor TFIIS.

The Journal of cell biology ·Vol. 143 ·No. 6 ·1998-12-14 ·Pages 1447-55

Albertini M, Pemberton LF, Rosenblum JS, Blobel G

Abstract

We have identified a novel pathway for protein import into the nucleus. We have shown that the previously identified but uncharacterized yeast protein Nmd5p functions as a karyopherin. It was therefore designated Kap119p (karyopherin with Mr of 119 kD). We localized Kap119p to both the nucleus and the cytoplasm. We identified the transcription elongation factor TFIIS as its major cognate import substrate. The cytoplasmic Kap119p exists as an approximately stoichiometric complex with TFIIS. RanGTP, not RanGDP, dissociated the isolated Kap119p/TFIIS complex and bound to Kap119p. Kap119p also bound directly to a number of peptide repeat containing nucleoporins in overlay assays. In wild-type cells, TFIIS was primarily localized to the nucleus. In a strain where KAP119 has been deleted, TFIIS was mislocalized to the cytoplasm indicating that TFIIS is imported into the nucleus by Kap119p. The transport of various substrates that use other karyopherin-mediated import or export pathways was not affected in a kap119Delta strain. Hence Kap119p is a novel karyopherin that is responsible for the import of the transcription elongation factor TFIIS.

MeSH Terms
Cell Nucleus/metabolism Cytoplasm/metabolism Fungal Proteins/metabolism GTP-Binding Proteins/metabolism Gene Deletion Genes, Fungal Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Kinetics Nuclear Proteins/chemical synthesis,genetics,metabolism Recombinant Proteins/metabolism Saccharomyces cerevisiae/genetics,metabolism Substrate Specificity Transcription Factors/isolation & purification,metabolism Transcription Factors, General Transcriptional Elongation Factors ran GTP-Binding Protein
Chemicals
Fungal Proteins Nuclear Proteins Recombinant Proteins Transcription Factors Transcription Factors, General Transcriptional Elongation Factors transcription factor S-II Guanosine Diphosphate Guanosine Triphosphate GTP-Binding Proteins ran GTP-Binding Protein
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Albertini M
Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, New York, New York 10021, USA.
Pemberton L F
Rosenblum J S
Blobel G
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.143.6.1447
Published
1998-12-14
Pages
1447-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2132971
Subset
IM
Grants
NIGMS NIH HHS · GM-18720 · United States
External Links
PubMed source DOI: 10.1083/jcb.143.6.1447 PMC Full Text View journal details
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