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PMID: 9819423 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell cycle regulation of the Saccharomyces cerevisiae polo-like kinase cdc5p.

Molecular and cellular biology ·Vol. 18 ·No. 12 ·1998-12-00 ·Pages 7360-70

Cheng L, Hunke L, Hardy CF

Abstract

Progression through and completion of mitosis require the actions of the evolutionarily conserved Polo kinase. We have determined that the levels of Cdc5p, a Saccharomyces cerevisiae member of the Polo family of mitotic kinases, are cell cycle regulated. Cdc5p accumulates in the nuclei of G2/M-phase cells, and its levels decline dramatically as cells progress through anaphase and begin telophase. We report that Cdc5p levels are sensitive to mutations in key components of the anaphase-promoting complex (APC). We have determined that Cdc5p-associated kinase activity is restricted to G2/M and that this activity is posttranslationally regulated. These results further link the actions of the APC to the completion of mitosis and suggest possible roles for Cdc5p during progression through and completion of mitosis.

MeSH Terms
Anaphase-Promoting Complex-Cyclosome Cell Cycle/physiology Cell Cycle Proteins Cell Nucleus/metabolism Flow Cytometry Fluorescent Antibody Technique Fungal Proteins/metabolism Ligases/physiology Mitosis/physiology Phosphorylation Protein Kinases/metabolism Protein Processing, Post-Translational/physiology Protein Serine-Threonine Kinases Saccharomyces cerevisiae/enzymology Saccharomyces cerevisiae Proteins Transformation, Genetic/genetics Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases
Chemicals
Cell Cycle Proteins Fungal Proteins Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Protein Kinases Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae Ligases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cheng L
Department of Cell Biology and Physiology and Department of Genetics, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
Hunke L
Hardy C F
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.18.12.7360
Published
1998-12-00
Pages
7360-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC109318
Subset
IM
Grants
NIGMS NIH HHS · GM5678801 · United States
External Links
PubMed source DOI: 10.1128/MCB.18.12.7360 PMC Full Text View journal details
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