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PMID: 9482730 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The polo-like kinase Plx1 is required for M phase exit and destruction of mitotic regulators in Xenopus egg extracts.

The EMBO journal ·Vol. 17 ·No. 5 ·1998-03-02 ·Pages 1328-35

Descombes P, Nigg EA

Abstract

Polo-like kinases (Plks), named after the Drosophila gene product polo, have been implicated in the regulation of multiple aspects of mitotic progression, including the activation of the Cdc25 phosphatase, bipolar spindle formation and cytokinesis. Genetic analyses performed in yeast and Drosophila suggest a function for Plks at late stages of mitosis, but biochemical data to support such a function in vertebrate organisms are lacking. Here we have taken advantage of Xenopus egg extracts for exploring the function of Plx1, a Xenopus Plk, during the cell cycle transition from M phase to interphase (I phase). We found that the addition of a catalytically inactive Plx1 mutant to M phase-arrested egg extracts blocked their Ca2+-induced release into interphase. Concomitantly, the proteolytic destruction of several targets of the anaphase-promoting complex and the inactivation of the Cdc2 protein kinase (Cdk1) were prevented. Moreover, the M to I phase transition could be abolished by immunodepletion of Plx1, but was restored upon the addition of recombinant Plx1. These results demonstrate that the exit of egg extracts from M phase arrest requires active Plx1, and they strongly suggest an important role for Plx1 in the activation of the proteolytic machinery that controls the exit from mitosis.

MeSH Terms
Anaphase-Promoting Complex-Cyclosome Animals CDC2 Protein Kinase/metabolism Calcium/pharmacology Cell Cycle Proteins/metabolism Cell Extracts Cyclin B/metabolism Drosophila Proteins Ligases/metabolism Mitosis/physiology Mutation Ovum Protein Serine-Threonine Kinases/genetics,metabolism,pharmacology Proto-Oncogene Proteins c-mos/metabolism Recombinant Fusion Proteins Schizosaccharomyces pombe Proteins Securin Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Xenopus Xenopus Proteins
Chemicals
Cell Cycle Proteins Cell Extracts Cut2 protein, S pombe CycB protein, Drosophila Cyclin B Drosophila Proteins Recombinant Fusion Proteins Schizosaccharomyces pombe Proteins Securin Xenopus Proteins Ubiquitin-Protein Ligase Complexes Anaphase-Promoting Complex-Cyclosome Ubiquitin-Protein Ligases Plk1 protein, Xenopus Protein Serine-Threonine Kinases Proto-Oncogene Proteins c-mos CDC2 Protein Kinase Ligases Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Descombes P
Department of Molecular Biology, University of Geneva, Science II, 30, quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland.
Nigg E A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.5.1328
Published
1998-03-02
Pages
1328-35
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170481
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/17.5.1328 PMC Full Text View journal details
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