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PMID: 8943332 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A novel role for Cdc5p in DNA replication.

Molecular and cellular biology ·Vol. 16 ·No. 12 ·1996-12-00 ·Pages 6775-82

Hardy CF, Pautz A

Abstract

DNA replication initiates from specific chromosomal sites called origins, and in the budding yeast Saccharomyces cerevisiae these sites are occupied by the origin recognition complex (ORC). Dbf4p is proposed to play a role in targeting the G1/S kinase Cdc7p to initiation complexes late in G1. We report that Dbf4p may also recruit Cdc5p to origin complexes. Cdc5p is a member of the Polo family of kinases that is required for the completion of mitosis. Cdc5p and Cdc7p each interact with a distinct domain of Dbf4p. cdc5-1 mutants have a plasmid maintenance defect that can be suppressed by the addition of multiple origins. cdc5-1 orc2-1 double mutants are synthetically lethal. Levels of Cdc5p were found to be cell cycle regulated and peaked in G2/M. These results suggest a role for Cdc5p and possibly Polo-like kinases at origin complexes.

MeSH Terms
Cell Cycle Proteins DNA Replication DNA, Fungal/genetics Gene Expression Regulation, Fungal Protein Kinases/genetics Protein Serine-Threonine Kinases Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins
Chemicals
Cell Cycle Proteins DNA, Fungal Saccharomyces cerevisiae Proteins Protein Kinases Protein Serine-Threonine Kinases CDC5 protein, S cerevisiae
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hardy C F
Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, Missouri 63110, USA. chardy@cellbio.wustl.edu
Pautz A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.16.12.6775
Email
chardy@cellbio.wustl.edu
Published
1996-12-00
Pages
6775-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC231680
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.16.12.6775 PMC Full Text View journal details
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