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PMID: 9770487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A DNA damage and stress inducible G protein-coupled receptor blocks cells in G2/M.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 21 ·1998-10-13 ·Pages 12334-9

Weng Z, Fluckiger AC, Nisitani S, Wahl MI, Le LQ, Hunter CA, Fernal AA, Le Beau MM, Witte ON

Abstract

Cell cycle progression is monitored by highly coordinated checkpoint machinery, which is activated to induce cell cycle arrest until defects like DNA damage are corrected. We have isolated an anti-proliferative cell cycle regulator named G2A (for G2 accumulation), which is predominantly expressed in immature T and B lymphocyte progenitors and is a member of the seven membrane-spanning G protein-coupled receptor family. G2A overexpression attenuates the transformation potential of BCR-ABL and other oncogenes, and leads to accumulation of cells at G2/M independently of p53 and c-Abl. G2A can be induced in lymphocytes and to a lesser extent in nonlymphocyte cell lines or tissues by multiple stimuli including different classes of DNA-damaging agents and serves as a response to damage and cellular stimulation which functions to slow cell cycle progression.

MeSH Terms
3T3 Cells Amino Acid Sequence Animals Base Sequence Cell Cycle Proteins/chemistry,genetics,metabolism Cloning, Molecular DNA Damage DNA Primers DNA Replication G2 Phase GTP-Binding Proteins/metabolism Mice Mitosis Molecular Sequence Data Oxidative Stress Rats Receptors, G-Protein-Coupled Signal Transduction
Chemicals
Cell Cycle Proteins DNA Primers G2A receptor Receptors, G-Protein-Coupled GTP-Binding Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Weng Z
Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA.
Fluckiger A C
Nisitani S
Wahl M I
Le L Q
Hunter C A
Fernal A A
Le Beau M M
Witte O N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.21.12334
Published
1998-10-13
Pages
12334-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC22832
Subset
IM
Grants
NCI NIH HHS · P01 CA040046 · United States
NCI NIH HHS · CA40046 · United States
NCI NIH HHS · CA76204 · United States
Databases
GENBANK
AF083442
External Links
PubMed source DOI: 10.1073/pnas.95.21.12334 PMC Full Text View journal details
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