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PMID: 2251277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Mapping chromosome band 11q23 in human acute leukemia with biotinylated probes: identification of 11q23 translocation breakpoints with a yeast artificial chromosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 23 ·1990-12-00 ·Pages 9358-62

Rowley JD, Diaz MO, Espinosa R, Patel YD, van Melle E, Ziemin S, Taillon-Miller P, Lichter P, Evans GA, Kersey JH

Abstract

Translocations involving chromosome 11, band q23, are frequent recurring abnormalities in human acute lymphoblastic and acute myeloid leukemia. We used 19 biotin-labeled probes derived from genes and anonymous cosmids for hybridization to metaphase chromosomes from leukemia cells that contained four translocations involving band 11q23: t(4;11)(q21;q23), t(6;11)(q27;q23), t(9;11)(p22;q23), and t(11;19)(q23;p13). The location of the cosmid probes relative to the breakpoint in 11q23 was the same in all translocations. Of the cosmid clones containing known genes, CD3D was proximal and PBGD, THY1, SRPR, and ETS1 were distal to the breakpoint on 11q23. Hybridization of genomic DNA from a yeast clone containing yeast artificial chromosomes (YACs), that carry 320 kilobases (kb) of human DNA including CD3D and CD3G genes, showed that the YACs were split in all four translocations. These results indicate that the breakpoint at 11q23 in each of these translocations occurs within the 320 kb encompassed by these YACs; whether the breakpoint within the YACs is precisely the same in the different translocations is presently unknown.

MeSH Terms
Adult Base Sequence Biotin Cell Line Child Chromosome Banding Chromosome Mapping Chromosomes, Fungal Chromosomes, Human, Pair 11 Female Humans Karyotyping Leukemia, Myeloid, Acute/genetics Male Middle Aged Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics Saccharomyces cerevisiae/genetics Translocation, Genetic
Chemicals
Oligonucleotide Probes Biotin
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Rowley J D
Section of Hematology/Oncology, University of Chicago, IL 60637.
Diaz M O
Espinosa R
Patel Y D
van Melle E
Ziemin S
Taillon-Miller P
Lichter P
Evans G A
Kersey J H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.23.9358
Published
1990-12-00
Pages
9358-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55164
Subset
IM
Grants
NCI NIH HHS · CA 42557 · United States
NIGMS NIH HHS · GM 33868 · United States
NICHD NIH HHS · HD 18012 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.23.9358 PMC Full Text View journal details
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