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PMID: 2725497 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The BCR-ABL oncogene transforms Rat-1 cells and cooperates with v-myc.

Molecular and cellular biology ·Vol. 9 ·No. 3 ·1989-03-00 ·Pages 1263-70

Lugo TG, Witte ON

Abstract

The tyrosine kinase P210 is the gene product of the rearranged BCR-ABL locus on the Philadelphia chromosome (Ph1), which is found in leukemic cells of patients with chronic myelogenous leukemia. It has a weakly oncogenic effect in immature murine hematopoietic cells and does not transform NIH 3T3 cells. We have found that P210 has a strikingly different effect in Rat-1 cells, another line of established rodent fibroblasts. Stable expression of P210 in Rat-1 cells caused a distinct morphological change and conferred both tumorigenicity and capacity for anchorage-independent growth. The introduction of v-myc into Rat-1 cells expressing P210 led to complete morphological transformation and enhanced tumorigenicity. No such interaction took place in NIH 3T3 cells. Thus, Rat-1 cells can be used to detect cooperation between BCR-ABL and other oncogenes and may prove useful for the identification of secondary oncogenic events in chronic myelogenous leukemia.

MeSH Terms
Abelson murine leukemia virus/genetics Animals Cell Line Cell Transformation, Neoplastic Humans Leukemia, Experimental/genetics,pathology Leukemia, Myelogenous, Chronic, BCR-ABL Positive/genetics Mice Oncogene Proteins, Viral/biosynthesis,genetics Oncogenes Rats
Chemicals
Oncogene Proteins, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lugo T G
Department of Microbiology, University of California, Los Angeles 90024-1570.
Witte O N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.3.1263-1270.1989
Published
1989-03-00
Pages
1263-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362717
Subset
IM
Grants
NIGMS NIH HHS · 5T32GM08243-01 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.3.1263-1270.1989 PMC Full Text View journal details
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