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PMID: 3540951 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Overlapping cDNA clones define the complete coding region for the P210c-abl gene product associated with chronic myelogenous leukemia cells containing the Philadelphia chromosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 24 ·1986-12-00 ·Pages 9768-72

Mes-Masson AM, McLaughlin J, Daley GQ, Paskind M, Witte ON

Abstract

The Philadelphia chromosome, observed in greater than 90% of patients with chronic myelogenous leukemia, results from a reciprocal translocation between chromosomes 9 and 22. The translocation breakpoint on chromosome 9 occurs near the ABL gene and correlates with the production of a chronic myelogenous leukemia-specific 8.5-kilobase ABL-related mRNA species accompanied by a structurally altered ABL protein (P210c-abl). The N-terminal sequence of the protein is derived from the BCR gene on chromosome 22. We have isolated overlapping cDNA clones from the K-562 cell line corresponding to approximately 8.5 kilobases of mRNA and have sequenced 2550 nucleotides at the 5' end. Our results indicate that the 5' end of the 8.5-kilobase mRNA consists of greater than 400 nucleotides of noncoding sequence that are greater than 80% G + C rich. Based on our sequence analysis, we propose that initiation of translation occurs at nucleotide 471, such that the initial 927 amino acids of P210c-abl are derived from BCR sequences. Our cDNA clones thus define the complete coding sequences for the P210c-abl gene product.

MeSH Terms
Base Sequence Cell Line Cloning, Molecular DNA/genetics Humans Leukemia, Myeloid/genetics Philadelphia Chromosome Proto-Oncogene Proteins/genetics Proto-Oncogenes RNA, Messenger/genetics
Chemicals
Proto-Oncogene Proteins RNA, Messenger DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mes-Masson A M
McLaughlin J
Daley G Q
Paskind M
Witte O N
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.24.9768
Published
1986-12-00
Pages
9768-72
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC387222
Subset
IM
Databases
GENBANK
M15025
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1073/pnas.83.24.9768 PMC Full Text View journal details
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