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PMID: 9746578 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Contact-dependent protein secretion in Porphyromonas gingivalis.

Infection and immunity ·Vol. 66 ·No. 10 ·1998-10-00 ·Pages 4777-82

Park Y, Lamont RJ

Abstract

Porphyromonas gingivalis can induce its uptake by host epithelial cells; however, the nature and role of the P. gingivalis molecules involved in this invasion process have yet to be determined. In this study, modulation of secreted P. gingivalis proteins following association with gingival epithelial cells was investigated. Western immunoblot analysis showed that contact with epithelial cells or epithelial cell growth media induces P. gingivalis 33277 to secrete several proteins with molecular masses between 35 and 95 kDa. Secretion of the Arg-gingipain and Lys-gingipain proteases was repressed under these conditions. The contact-induced secreted protein profile was altered in Arg-gingipain-deficient and Lys-gingipain-deficient mutants, indicating a possible role for these proteases in the secretion pathway. The P. gingivalis contact-dependent protein secretion pathway differs to some extent from type III protein secretion pathways in enteric pathogens, as a gene homologous to the invA family genes was not detected in P. gingivalis. The secreted proteins of P. gingivalis may play a role in the interactions of the organism with host cells.

MeSH Terms
Adhesins, Bacterial/chemistry,genetics,metabolism Bacterial Adhesion Bacterial Proteins/analysis,chemistry,genetics,metabolism Cysteine Endopeptidases/chemistry,genetics,physiology Epithelial Cells/microbiology Fimbriae Proteins Genes, Bacterial Gingipain Cysteine Endopeptidases Gingiva/cytology,microbiology Hemagglutinins/chemistry,genetics,physiology Mutagenesis, Insertional Porphyromonas gingivalis/genetics,metabolism Sequence Analysis Signal Transduction
Chemicals
Adhesins, Bacterial Bacterial Proteins Gingipain Cysteine Endopeptidases Hemagglutinins fimbrillin invA protein, Bacteria Fimbriae Proteins Cysteine Endopeptidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Park Y
Department of Oral Biology, School of Dentistry, University of Washington, Seattle, Washington 98195, USA.
Lamont R J
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.66.10.4777-4782.1998
Published
1998-10-00
Pages
4777-82
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC108589
Subset
IM
Grants
NIDCR NIH HHS · R01 DE011111 · United States
NIDCR NIH HHS · R37 DE011111 · United States
NIDCR NIH HHS · DE11111 · United States
External Links
PubMed source DOI: 10.1128/IAI.66.10.4777-4782.1998 PMC Full Text View journal details
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