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PMID: 7781600 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Contact of Shigella with host cells triggers release of Ipa invasins and is an essential function of invasiveness.

The EMBO journal ·Vol. 14 ·No. 11 ·1995-06-01 ·Pages 2461-70

Watarai M, Tobe T, Yoshikawa M, Sasakawa C

Abstract

The invasion of colonic epithelial cells by Shigella, an early essential step for causing bacillary dysentery, is mediated by the IpaB, IpaC and IpaD proteins. Secretion of the Ipa proteins from Shigella requires functions encoded by the mxi and spa loci. In this study, we show that contact between the bacteria and epithelial cell triggers release of the Ipa proteins into the external medium, which results in a rapid decrease in levels of Ipa proteins presented on the cell surface. When the bacteria were used to infect polarized Caco-2 cells, release of Ipa proteins occurred efficiently from bacteria interacting with the basolateral surface rather than with the apical surface. Moreover, the interaction of bacteria with components of the extracellular matrix, such as fibronectin, laminin or collagen type IV, also stimulates the release of Ipa proteins. The release of Ipa proteins from Shigella required the surface-located Spa32 protein encoded by one of the spa genes on the large plasmid.

Related Genes
mxi spa
MeSH Terms
Adhesins, Bacterial Animals Bacterial Adhesion/genetics,physiology Bacterial Proteins/genetics,metabolism Base Sequence Colon/microbiology DNA Primers/genetics DNA, Bacterial/genetics Dysentery, Bacillary/etiology Epithelium/microbiology Extracellular Matrix/microbiology Genes, Bacterial Humans Molecular Sequence Data Sequence Deletion Shigella flexneri/genetics,pathogenicity,physiology Virulence/physiology
Chemicals
Adhesins, Bacterial Bacterial Proteins DNA Primers DNA, Bacterial invasin, Yersinia
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Watarai M
Department of Bacteriology, University of Tokyo, Japan.
Tobe T
Yoshikawa M
Sasakawa C
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44 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-06-01
Pages
2461-70
Language
English
Region
England
NLM ID
8208664
PMCID
PMC398359
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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