Limited N-terminal sequence analysis.

Methods in enzymology ·Vol. 182 ·1990-00-00 ·Pages 602-13

Matsudaira P

Abstract

Current methods in protein purification permit sequence analysis of picomole quantities of proteins and peptides. The key to obtaining sequence is to choose as the last step in the purification protocol the simplest (usually the fastest) method for isolating the protein or peptide.

MeSH Terms

Amino Acid Sequence Chromatography, High Pressure Liquid/methods Electrophoresis, Polyacrylamide Gel/methods Indicators and Reagents Methods Peptide Fragments/isolation & purification Peptide Hydrolases Peptides/isolation & purification Proteins/isolation & purification Staining and Labeling

Chemicals

Indicators and Reagents Peptide Fragments Peptides Proteins Peptide Hydrolases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Matsudaira P

Article Info

Journal

Methods in enzymology

Abbr.

Methods Enzymol

ISSN

0076-6879

DOI

10.1016/0076-6879(90)82047-6

Published

1990-00-00

Pages

602-13

Language

English

Region

United States

NLM ID

0212271

Subset

External Links

PubMed source DOI: 10.1016/0076-6879(90)82047-6 View journal details

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