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PMID: 9284114 Published · ppublish English Journal Article

Porphyromonas gingivalis lipopolysaccharide-stimulated bone resorption via CD14 is inhibited by broad-spectrum antibiotics.

Infection and immunity ·Vol. 65 ·No. 9 ·1997-09-00 ·Pages 3513-9

Miyata Y, Takeda H, Kitano S, Hanazawa S

Abstract

In the present study, we examined mechanisms of Porphyromonas gingivalis lipopolysaccharide (P-LPS)-stimulated bone resorption via CD14, one of the lipopolysaccharide (LPS) receptors, and also assessed the inhibitory action of several kinds of antibiotics on the LPS-induced stimulation. First, we observed by using mouse embryonic calvarial cells that P-LPS stimulated bone resorption through the action of endogenous interleukin-1beta (IL-1beta) and interleukin-6 (IL-6) via CD14 because (i) P-LPS-stimulated expression of IL-1beta and IL-6 genes in calvarial cells was inhibited by an anti-mouse CD14 antibody, (ii) stimulated bone resorption was markedly inhibited by both IL-1beta and IL-6 antibodies, and (iii) P-LPS-stimulated bone resorption was clearly neutralized by an anti-mouse CD14 antibody. Next, we examined the effects of several kinds of antibiotics on P-LPS-stimulated bone resorption via CD14. Two of them, chloramphenicol and erythromycin, inhibited P-LPS-stimulated bone resorption in a dose-dependent manner. In an additional experiment, we observed that chloramphenicol clearly inhibited P-LPS-stimulated expression of the CD14, IL-1beta, and IL-6 genes in calvarial cells. These results suggest that chloramphenicol might be a useful antibiotic as an anti-inflammatory agent against P-LPS-stimulated periodontal destruction occurring via CD14 in periodontal disease.

MeSH Terms
Ampicillin/pharmacology Animals Anti-Bacterial Agents/pharmacology Bone Resorption Chloramphenicol/pharmacology Erythromycin/pharmacology Gene Expression/drug effects Interleukin-1/physiology Interleukin-6/physiology Lipopolysaccharide Receptors/physiology Lipopolysaccharides/pharmacology Mice Osteoclasts/metabolism Porphyromonas gingivalis/pathogenicity RNA, Messenger/genetics Skull Streptomycin/pharmacology Tetracycline/pharmacology
Chemicals
Anti-Bacterial Agents Interleukin-1 Interleukin-6 Lipopolysaccharide Receptors Lipopolysaccharides RNA, Messenger Erythromycin Chloramphenicol Ampicillin Tetracycline Streptomycin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miyata Y
Department of Oral Microbiology, Meikai University School of Dentistry, Sakado City, Saitama, Japan.
Takeda H
Kitano S
Hanazawa S
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.65.9.3513-3519.1997
Published
1997-09-00
Pages
3513-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC175501
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.65.9.3513-3519.1997 PMC Full Text View journal details
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