Expression of monocyte chemoattractant protein 1 (MCP-1) in adult periodontal disease: increased monocyte chemotactic activity in crevicular fluids and induction of MCP-1 expression in gingival tissues.

Infection and immunity ·Vol. 61 ·No. 12 ·1993-12-00 ·Pages 5219-24

Hanazawa S, Kawata Y, Takeshita A, Kumada H, Okithu M, Tanaka S, Yamamoto Y, Masuda T, Umemoto T, Kitano S

Abstract

The present study shows that monocyte chemotactic activity in crevicular fluids increases with severity of the disease and that a monocyte chemoattractant, monocyte chemoattractant protein 1 (MCP-1), is expressed as the predominant cytokine of gingival tissues and their fibroblasts treated with Porphyromonas (Bacteroides) gingivalis lipopolysaccharide (P-LPS). High monocyte chemotactic activity in the crevicular fluids was neutralized significantly by antiserum specific for the JE/MCP-1 protein. Marked expression of the MCP-1 gene was observed in the gingival tissues of all adult periodontal patients tested, but not in those of healthy subjects. Monocyte chemotactic activity was observed in culture supernatants of human normal gingival tissues treated with P-LPS, and the chemotactic activity increased in a dose-related manner. Expression of MCP-1 in P-LPS-treated human gingival fibroblasts was further examined. P-LPS induced the MCP-1 gene expression in a dose- and treatment time-dependent manner. The MCP-1 gene product in the culture supernatant was detected as two forms with molecular masses of 11,000 and 15,000 Da by immunoprecipitation with the specific antiserum. The MCP-1 gene expression was induced in the fibroblasts treated with interleukin-1 beta and tumor necrosis factor alpha, but not with interleukin-6. These results suggest that gingival fibroblasts can participate in monocyte recruitment in gingival tissues of adult periodontal patients via the MCP-1 gene product and that MCP-1 plays an important role in the inflammatory reaction in the disease.

Related Genes

MCP-1

MeSH Terms

Adult Antibodies Chemokine CCL2 Chemotactic Factors/genetics,metabolism Chemotaxis, Leukocyte Cytokines/pharmacology Gene Expression/drug effects Gingiva/drug effects,immunology,pathology Gingival Crevicular Fluid/immunology Humans In Vitro Techniques Lipopolysaccharides/pharmacology Monocytes/immunology,pathology Neutralization Tests Periodontal Diseases/genetics,immunology,pathology Porphyromonas gingivalis/immunology

Chemicals

Antibodies Chemokine CCL2 Chemotactic Factors Cytokines Lipopolysaccharides

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Hanazawa S

Department of Oral Microbiology, Meikai University School of Dentistry, Saitama, Japan.

Kawata Y

Takeshita A

Kumada H

Okithu M

Tanaka S

Yamamoto Y

Masuda T

Umemoto T

Kitano S

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35 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.61.12.5219-5224.1993

Published

1993-12-00

Pages

5219-24

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC281304

Subset

External Links

PubMed source DOI: 10.1128/iai.61.12.5219-5224.1993 PMC Full Text View journal details

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Expression of monocyte chemoattractant protein 1 (MCP-1) in adult periodontal disease: increased monocyte chemotactic activity in crevicular fluids and induction of MCP-1 expression in gingival tissues.

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