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PMID: 9724822 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Protein kinase CK2 interacts with and phosphorylates the Arabidopsis circadian clock-associated 1 protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 95 ·No. 18 ·1998-09-01 ·Pages 11020-5

Sugano S, Andronis C, Green RM, Wang ZY, Tobin EM

Abstract

The circadian clock-associated 1 (CCA1) gene encodes a Myb-related transcription factor that has been shown to be involved in the phytochrome regulation of Lhcb1*3 gene expression and in the function of the circadian oscillator in Arabidopsis thaliana. By using a yeast interaction screen to identify proteins that interact with CCA1, we have isolated a cDNA clone encoding a regulatory (beta) subunit of the protein kinase CK2 and have designated it as CKB3. CKB3 is the only reported example of a third beta-subunit of CK2 found in any organism. CKB3 interacts specifically with CCA1 both in a yeast two-hybrid system and in an in vitro interaction assay. Other subunits of CK2 also show an interaction with CCA1 in vitro. CK2 beta-subunits stimulate binding of CCA1 to the CCA1 binding site on the Lhcb1*3 gene promoter, and recombinant CK2 is able to phosphorylate CCA1 in vitro. Furthermore, Arabidopsis plant extracts contain a CK2-like activity that affects the formation of a DNA-protein complex containing CCA1. These results suggest that CK2 can modulate CCA1 activity both by direct interaction and by phosphorylation of the CCA1 protein and that CK2 may play a role in the function of CCA1 in vivo.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,metabolism Arabidopsis Proteins Base Sequence Casein Kinase II Circadian Rhythm DNA, Complementary DNA, Recombinant DNA-Binding Proteins/genetics,metabolism Molecular Sequence Data Phosphorylation Promoter Regions, Genetic Protein Binding Protein Serine-Threonine Kinases/genetics,metabolism Recombinant Proteins/genetics,metabolism Sequence Homology, Amino Acid Transcription Factors/chemistry,genetics,metabolism
Chemicals
Arabidopsis Proteins CCA1 protein, Arabidopsis DNA, Complementary DNA, Recombinant DNA-Binding Proteins Recombinant Proteins Transcription Factors Casein Kinase II Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Sugano S
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90095-1606, USA.
Andronis C
Green R M
Wang Z Y
Tobin E M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.95.18.11020
Published
1998-09-01
Pages
11020-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC28013
Subset
IM
Grants
NIGMS NIH HHS · R01 GM023167 · United States
NIGMS NIH HHS · R01-GM-23167 · United States
Databases
GENBANK
AF068318
External Links
PubMed source DOI: 10.1073/pnas.95.18.11020 PMC Full Text View journal details
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