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PMID: 9687505 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Regulation of the Cln3-Cdc28 kinase by cAMP in Saccharomyces cerevisiae.

The EMBO journal ·Vol. 17 ·No. 15 ·1998-08-03 ·Pages 4370-8

Hall DD, Markwardt DD, Parviz F, Heideman W

Abstract

The yeast Saccharomyces cerevisiae grows at widely varying rates in different growth media. In order to maintain a relatively constant cell size, yeast cells must regulate the rate of progress through the cell cycle to match changes in growth rate, moving quickly through G1 in rich medium, and slowly in poor medium. We have examined connections between nutrients, and the expression and activity of Cln3-Cdc28 kinase that regulates the G1-S boundary of the cell cycle in yeast, a point referred to as Start. We find that Cln3 protein levels are highest in glucose and lower in poorer carbon sources. This regulation involves both transcriptional and post-transcriptional control. Although the Ras-cAMP pathway does not appear to affect CLN3 transcription, cAMP increases Cln3 protein levels and Cln3-Cdc28 kinase activity. This regulation requires untranslated regions of the CLN3 message, and can be explained by changes in protein synthesis rates caused by cAMP. A model for CLN3 regulation and function is presented in which CLN3 regulates G1 length in response to nutrients.

MeSH Terms
CDC28 Protein Kinase, S cerevisiae/metabolism Carbon/metabolism Cyclic AMP/physiology Cyclins/biosynthesis,metabolism,physiology Cycloheximide/pharmacology Fungal Proteins/biosynthesis,metabolism,physiology G1 Phase/physiology Saccharomyces cerevisiae/cytology,enzymology,physiology Saccharomyces cerevisiae Proteins
Chemicals
CLN3 protein, S cerevisiae Cyclins Fungal Proteins Saccharomyces cerevisiae Proteins Carbon Cycloheximide Cyclic AMP CDC28 Protein Kinase, S cerevisiae Cln3-Cdc28 kinase complex, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hall D D
Department of Biomolecular Chemistry, University of Wisconsin, Madison 53706, USA.
Markwardt D D
Parviz F
Heideman W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.15.4370
Published
1998-08-03
Pages
4370-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170770
Subset
IM
Grants
NIGMS NIH HHS · GM42406 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.15.4370 PMC Full Text View journal details
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