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PMID: 2017169 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The CDC25 protein of Saccharomyces cerevisiae promotes exchange of guanine nucleotides bound to ras.

Molecular and cellular biology ·Vol. 11 ·No. 5 ·1991-05-00 ·Pages 2641-6

Jones S, Vignais ML, Broach JR

Abstract

The product of the CDC25 gene of Saccharomyces cerevisiae, in its capacity as an activator of the RAS/cyclic AMP pathway, is required for initiation of the cell cycle. In this report, we provide an identification of Cdc25p, the product of the CDC25 gene, and evidence that it promotes exchange of guanine nucleotides bound to Ras in vitro. Extracts of strains containing high levels of Cdc25p catalyze both removal of GDP from and the concurrent binding of GTP to Ras. This same activity is also obtained with an immunopurified Cdc25p-beta-galactosidase fusion protein, suggesting that Cdc25p participates directly in the exchange reaction. This biochemical activity is consistent with previous genetic analysis of CDC25 function.

MeSH Terms
Cell Cycle Proteins Cell Membrane/metabolism Fungal Proteins/genetics,isolation & purification,metabolism Genotype Guanosine Diphosphate/metabolism Guanosine Triphosphate/metabolism Kinetics Molecular Weight Protein Binding Recombinant Fusion Proteins/isolation & purification,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins ras Proteins ras-GRF1
Chemicals
CDC25 protein, S cerevisiae Cell Cycle Proteins Fungal Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins ras-GRF1 Guanosine Diphosphate Guanosine Triphosphate ras Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jones S
Department of Molecular Biology, Princeton University, New Jersey 08544.
Vignais M L
Broach J R
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27 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.5.2641-2646.1991
Published
1991-05-00
Pages
2641-6
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360033
Subset
IM
Grants
NCI NIH HHS · CA41086 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.5.2641-2646.1991 PMC Full Text View journal details
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