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PMID: 9564047 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Dbp5p, a cytosolic RNA helicase, is required for poly(A)+ RNA export.

The EMBO journal ·Vol. 17 ·No. 9 ·1998-05-01 ·Pages 2651-62

Tseng SS, Weaver PL, Liu Y, Hitomi M, Tartakoff AM, Chang TH

Abstract

The DBP5 gene encodes a putative RNA helicase of unknown function in the yeast Saccharomyces cerevisiae. It is shown here that Dbp5p is an ATP-dependent RNA helicase required for polyadenylated [poly(A)+] RNA export. Surprisingly, Dbp5p is present predominantly, if not exclusively, in the cytoplasm, and is highly enriched around the nuclear envelope. This observation raises the possibility that Dbp5p may play a role in unloading or remodeling messenger RNA particles (mRNPs) upon arrival in the cytoplasm and in coupling mRNP export and translation. The functions of Dbp5p are likely to be conserved, since its potential homologues can be found in a variety of eukaryotic cells.

MeSH Terms
Amino Acid Sequence Animals Cell Line Cytoplasm/enzymology Dictyostelium/enzymology Drosophila/embryology,enzymology Embryo, Nonmammalian/enzymology Evolution, Molecular Female Genomic Library Humans Jurkat Cells Mice Molecular Sequence Data Nuclear Envelope/enzymology Oocytes/physiology PC12 Cells Phylogeny RNA Helicases RNA Nucleotidyltransferases/chemistry,genetics,metabolism RNA, Messenger/metabolism Rats Recombinant Proteins/metabolism Saccharomyces cerevisiae/enzymology Schizosaccharomyces/enzymology Sequence Alignment Sequence Homology, Amino Acid Subcellular Fractions/enzymology Xenopus
Chemicals
RNA, Messenger Recombinant Proteins RNA Nucleotidyltransferases RNA Helicases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tseng S S
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210, USA.
Weaver P L
Liu Y
Hitomi M
Tartakoff A M
Chang T H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.9.2651
Published
1998-05-01
Pages
2651-62
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170606
Subset
IM
Grants
NIGMS NIH HHS · GM48752 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.9.2651 PMC Full Text View journal details
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