Home LiteratureArticle Details
PMID: 9305650 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins.

The EMBO journal ·Vol. 16 ·No. 16 ·1997-08-15 ·Pages 5086-97

Teixeira MT, Siniossoglou S, Podtelejnikov S, Bénichou JC, Mann M, Dujon B, Hurt E, Fabre E

Abstract

Nup145p is an essential yeast nucleoporin involved in nuclear export of polyadenylated RNAs. We demonstrate here that Nup145p is cleaved in vivo to yield two functionally distinct domains: a carboxy-terminal domain (C-Nup145p) which is located at the nuclear pore complex (NPC) and assembles into the Nup84p complex, and a GLFG-containing amino-terminal domain (N-Nup145p) which is not part of this complex. Whereas the essential C-Nup145p accomplishes the functions required for efficient mRNA export and normal NPC distribution, N-Nup145p, which is homologous to the GLFG-containing nucleoporins Nup100p and Nup116p, is not necessary for cell growth. However, the N-Nup145p becomes essential in a nup188 mutant background. Strikingly, generation of a free N-domain is a prerequisite for complementation of this peculiar synthetic lethal mutant. These data suggest that N- and C-domains of Nup145p perform independent functions, and that the in vivo cleavage observed is of functional importance.

MeSH Terms
Amino Acid Sequence Biological Transport/physiology Blotting, Western Electrophoresis, Polyacrylamide Gel Fungal Proteins/analysis,chemistry,genetics,metabolism Microscopy, Electron Molecular Sequence Data Mutagenesis, Site-Directed/genetics Nuclear Envelope/chemistry,metabolism Nuclear Pore Complex Proteins Nuclear Proteins/metabolism Protein Processing, Post-Translational RNA, Fungal/metabolism RNA, Messenger/metabolism RNA-Binding Proteins/analysis,chemistry,genetics,metabolism Recombinant Fusion Proteins/chemistry,metabolism Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Staphylococcal Protein A/genetics Yeasts/genetics,growth & development,metabolism
Chemicals
Fungal Proteins NUP145 protein, S cerevisiae NUP84 protein, S cerevisiae Nuclear Pore Complex Proteins Nuclear Proteins RNA, Fungal RNA, Messenger RNA-Binding Proteins Recombinant Fusion Proteins Saccharomyces cerevisiae Proteins Staphylococcal Protein A
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Teixeira M T
Unité de Génétique Moléculaire des levures (URA 1300 CNRS and UPR 927 Univ. P M Curie), Institut Pasteur, Département des Biotechnologies, Paris, France.
Siniossoglou S
Podtelejnikov S
Bénichou J C
Mann M
Dujon B
Hurt E
Fabre E
References (30)
30 references, click to expand
  1. Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.
    Nature. 1996 Feb 1;379(6564):466-9 PMID: 8559255
  2. The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1699-713 PMID: 8557738
  3. Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p.
    J Cell Biol. 1996 Jun;133(6):1141-52 PMID: 8682854
  4. The yeast nucleoporin Nup188p interacts genetically and physically with the core structures of the nuclear pore complex.
    J Cell Biol. 1996 Jun;133(6):1153-62 PMID: 8682855
  5. C-terminal truncations of the yeast nucleoporin Nup145p produce a rapid temperature-conditional mRNA export defect and alterations to nuclear structure.
    Mol Cell Biol. 1997 Feb;17(2):906-20 PMID: 9001245
  6. Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells.
    J Cell Biol. 1997 Feb 24;136(4):747-59 PMID: 9049242
  7. Defining the essential functional regions of the nucleoporin Nup145p.
    J Cell Sci. 1997 Apr;110 ( Pt 7):911-25 PMID: 9133678
  8. A family of versatile centromeric vectors designed for use in the sectoring-shuffle mutagenesis assay in Saccharomyces cerevisiae.
    Gene. 1988 Oct 30;70(2):303-12 PMID: 3063604
  9. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain.
    Cell. 1990 Jun 15;61(6):979-89 PMID: 2112428
  10. High efficiency transformation of intact yeast cells by electric field pulses.
    Biotechnology (N Y). 1990 Mar;8(3):223-7 PMID: 1366502
  11. One-step transformation of yeast in stationary phase.
    Curr Genet. 1992 Jan;21(1):83-4 PMID: 1735128
  12. Isolation and characterization of RAT1: an essential gene of Saccharomyces cerevisiae required for the efficient nucleocytoplasmic trafficking of mRNA.
    Genes Dev. 1992 Jul;6(7):1173-89 PMID: 1628825
  13. A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.
    EMBO J. 1992 Dec;11(13):5051-61 PMID: 1464327
  14. Novel proteases with unusual specificities.
    Curr Opin Cell Biol. 1992 Dec;4(6):1032-6 PMID: 1485956
  15. Heat shock proteins: molecular chaperones of protein biogenesis.
    Microbiol Rev. 1993 Jun;57(2):402-14 PMID: 8336673
  16. LexA cleavage and other self-processing reactions.
    J Bacteriol. 1993 Aug;175(16):4943-50 PMID: 8349538
  17. hnRNP proteins and the biogenesis of mRNA.
    Annu Rev Biochem. 1993;62:289-321 PMID: 8352591
  18. A temperature-sensitive NUP116 null mutant forms a nuclear envelope seal over the yeast nuclear pore complex thereby blocking nucleocytoplasmic traffic.
    J Cell Biol. 1993 Oct;123(2):275-84 PMID: 7691829
  19. NUP145 encodes a novel yeast glycine-leucine-phenylalanine-glycine (GLFG) nucleoporin required for nuclear envelope structure.
    J Cell Biol. 1994 Jun;125(5):955-69 PMID: 8195299
  20. Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p.
    J Cell Biol. 1994 Aug;126(3):619-30 PMID: 8045927
  21. A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution.
    EMBO J. 1994 Dec 15;13(24):6062-75 PMID: 7813444
  22. Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.
    EMBO J. 1995 Jan 3;14(1):76-87 PMID: 7828598
  23. Nuclear export signals and the fast track to the cytoplasm.
    Cell. 1995 Aug 11;82(3):341-4 PMID: 7634321
  24. Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins.
    Cell. 1995 Aug 11;82(3):485-94 PMID: 7634337
  25. A family of Ran binding proteins that includes nucleoporins.
    Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7525-9 PMID: 7638224
  26. The essential yeast nucleoporin NUP159 is located on the cytoplasmic side of the nuclear pore complex and serves in karyopherin-mediated binding of transport substrate.
    J Biol Chem. 1995 Aug 11;270(32):19017-21 PMID: 7642562
  27. Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
    J Cell Biol. 1995 Dec;131(5):1133-48 PMID: 8522578
  28. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins.
    Cell. 1995 Dec 1;83(5):683-92 PMID: 8521485
  29. Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
    J Cell Biol. 1995 Dec;131(6 Pt 2):1659-75 PMID: 8557736
  30. Nucleocytoplasmic transport.
    Science. 1996 Mar 15;271(5255):1513-8 PMID: 8599106
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/16.16.5086
Published
1997-08-15
Pages
5086-97
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170143
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/16.16.5086 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2