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PMID: 9564039 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Glucose sensing and signaling by two glucose receptors in the yeast Saccharomyces cerevisiae.

The EMBO journal ·Vol. 17 ·No. 9 ·1998-05-01 ·Pages 2566-73

Ozcan S, Dover J, Johnston M

Abstract

How eukaryotic cells sense availability of glucose, their preferred carbon and energy source, is an important, unsolved problem. Bakers' yeast (Saccharomyces cerevisiae) uses two glucose transporter homologs, Snf3 and Rgt2, as glucose sensors that generate a signal for induction of expression of genes encoding hexose transporters (HXT genes). We present evidence that these proteins generate an intracellular glucose signal without transporting glucose. The Snf3 and Rgt2 glucose sensors contain unusually long C-terminal tails that are predicted to be in the cytoplasm. These tails appear to be the signaling domains of Snf3 and Rgt2 because they are necessary for glucose signaling by Snf3 and Rgt2, and transplantation of the C-terminal tail of Snf3 onto the Hxt1 and Hxt2 glucose transporters converts them into glucose sensors that can generate a signal for glucose-induced HXT gene expression. These results support the idea that yeast senses glucose using two modified glucose transporters that serve as glucose receptors.

MeSH Terms
Amino Acid Sequence Base Sequence Consensus Sequence DNA Primers Gene Expression Regulation, Fungal Genotype Glucose/metabolism Membrane Proteins/chemistry,genetics,physiology Molecular Sequence Data Monosaccharide Transport Proteins/chemistry,genetics,physiology Polymerase Chain Reaction Receptors, Cell Surface/genetics,physiology Recombinant Proteins/biosynthesis,chemistry Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Sequence Alignment Signal Transduction beta-Galactosidase/biosynthesis
Chemicals
DNA Primers Membrane Proteins Monosaccharide Transport Proteins RGT2 protein, S cerevisiae Receptors, Cell Surface Recombinant Proteins SNF3 protein, S cerevisiae Saccharomyces cerevisiae Proteins glucose receptor beta-Galactosidase Glucose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ozcan S
Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA.
Dover J
Johnston M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/17.9.2566
Published
1998-05-01
Pages
2566-73
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1170598
Subset
IM
Grants
NIGMS NIH HHS · R01 GM032540 · United States
NIGMS NIH HHS · GM32540 · United States
External Links
PubMed source DOI: 10.1093/emboj/17.9.2566 PMC Full Text View journal details
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