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PMID: 8013905 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Altered regulatory responses to glucose are associated with a glucose transport defect in grr1 mutants of Saccharomyces cerevisiae.

Genetics ·Vol. 136 ·No. 4 ·1994-04-00 ·Pages 1279-85

Vallier LG, Coons D, Bisson LF, Carlson M

Abstract

The GRR1 gene of Saccharomyces cerevisiae affects glucose repression, cell morphology, divalent cation transport and other processes. We present a kinetic analysis showing that the grr1 mutant is also defective in high affinity glucose transport. In combination with a mutation in SNF3, a member of the glucose transporter gene family, grr1 strikingly impairs growth on glucose. These findings suggest that GRR1 and SNF3 affect glucose transport by distinct pathways. The mutation rgt1-1, a suppressor of snf3, restores both glucose transport and glucose repression to a grr1 mutant, but does not remedy the morphological defect. We suggest that GRR1 affects the glucose sensing process and that the association between transport and regulation may reflect the involvement of a transporter in glucose sensing.

Related Genes
SNF3 SUC2 grr1
MeSH Terms
Biological Transport/genetics Carrier Proteins/genetics DNA-Binding Proteins F-Box Proteins Fungal Proteins/genetics Genes, Fungal Glucose/metabolism Glycoside Hydrolases/genetics Mutation Nuclear Proteins Repressor Proteins Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins Ubiquitin-Protein Ligases beta-Fructofuranosidase
Chemicals
CYC8 protein, S cerevisiae Carrier Proteins DNA-Binding Proteins F-Box Proteins Fungal Proteins Nuclear Proteins Repressor Proteins Saccharomyces cerevisiae Proteins GRR1 protein, S cerevisiae Ubiquitin-Protein Ligases Glycoside Hydrolases beta-Fructofuranosidase Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vallier L G
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Coons D
Bisson L F
Carlson M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/136.4.1279
Published
1994-04-00
Pages
1279-85
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1205908
Subset
IM
Grants
NIGMS NIH HHS · GM34095 · United States
External Links
PubMed source DOI: 10.1093/genetics/136.4.1279 PMC Full Text View journal details
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