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PMID: 6394965 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Cloning of hexokinase isoenzyme PI from Saccharomyces cerevisiae: PI transformants confirm the unique role of hexokinase isoenzyme PII for glucose repression in yeasts.

Molecular & general genetics : MGG ·Vol. 198 ·No. 2 ·1984-00-00 ·Pages 50-4

Entian KD, Kopetzki E, Fröhlich KU, Mecke D

Abstract

Hexokinase isoenzyme PI was cloned using a gene pool obtained from a yeast strain having only one functional hexokinase, isoenzyme PI. The gene was characterized using 20 restriction enzymes and located within a region of 2.0 kbp. The PI plasmid strongly hybridized with the PII plasmids isolated previously (Fröhlich et al. 1984). Hence there was a close relationship between the two genes, one of which must have been derived from the other by gene duplication. In contrast, glucose repression was restored only in hexokinase PII transformants; PI transformants remained non-repressible. This observation provided additional evidence for the hypothesis of Entian (1980) that only hexokinase PII is necessary for glucose repression. Furthermore, glucose phosphorylating activity in PI transformants exceeded that of wild-type cells, giving clear evidence that the phosphorylating capacity is not important for glucose repression.

MeSH Terms
Cloning, Molecular Genes, Fungal Glucose/pharmacology Hexokinase/genetics Isoenzymes/genetics Plasmids Saccharomyces cerevisiae/drug effects,enzymology,genetics Transformation, Bacterial
Chemicals
Isoenzymes Hexokinase Glucose
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Entian K D
Kopetzki E
Fröhlich K U
Mecke D
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30 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00328699
Published
1984-00-00
Pages
50-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00328699
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