Inactivation by glucose of phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae.
Phosphoenolpyruvate carboxykinase showed high activity in Saccharomyces cerevisiae grown on gluconeogenic carbon sources. Addition of glucose to such cultures caused a rapid loss of the phosphoenolpyruvate carboxykinase activity. Fructose or mannose had the same effect as glucose, while 2-deoxyglucose or galactose were without effect. The inactivation was an irreversible process, since the regain of the activity was dependent of de novo protein synthesis. Cycloheximide did not prevent inactivation. All strains of the genus Saccharomyces tested showed inactivation of their phosphoenolpyruvate carboxykinase upon addition of glucose; this behaviour was not restricted to this genus.
17 references, click to expand
-
Effect of glucose on the activity and the kinetics of the maltose-uptake system and of alpha-glucosidase in Saccharomyces cerevisiae.Biochim Biophys Acta. 1969 Jul 30;184(2):299-305 PMID: 5809715
-
Isolation of a regulatory mutant of fructose-1,6-diphosphatase in Saccharomyces carlsbergensis.J Bacteriol. 1974 Mar;117(3):965-70 PMID: 4360542
-
A simplified method for determination of phosphoenolpyruvate carboxykinase activity.Anal Biochem. 1970 Mar;34:282-4 PMID: 5440912
-
Rapid loss of translatable messenger RNA of phosphoenolpyruvate carboxykinase during glucose repression in liver.Proc Natl Acad Sci U S A. 1974 Apr;71(4):1304-8 PMID: 4364533
-
On the activity and regulation of anaplerotic and gluconeogenetic enzymes during the growth process of baker's yeast. The biphasic growth.Eur J Biochem. 1975 Mar 3;52(1):1-7 PMID: 170081
-
RECIPROCAL EFFECTS OF CARBON SOURCES ON THE LEVELS OF AN AMP-SENSITIVE FRUCTOSE-1,6-DIPHOSPHATASE AND PHOSPHOFRUCTOKINASE IN YEAST.Biochem Biophys Res Commun. 1965 Jun 18;20:15-20 PMID: 14341932
-
A reinvestigation of the kinetic parameters of phosphoenolypyruvate carboxykinase.Biochem Biophys Res Commun. 1971 Nov 5;45(3):669-75 PMID: 5128176
-
Phosphoenolpyruvate carboxykinase in gluconeogenesis and its repression by hexoses.Biochem Biophys Res Commun. 1966 Jan 24;22(2):227-31 PMID: 5948139
-
Inactivation of fructose-1,6-diphosphatase by glucose in yeast.J Bacteriol. 1971 Aug;107(2):401-5 PMID: 4329729
-
Phosphoenolpyruvate carboxykinase from bakers' yeast. Isolation of the enzyme and study of its physical properties.J Biol Chem. 1970 Feb 25;245(4):792-8 PMID: 5416663
-
Concentrations of intermediary metabolites in yeast.Biochimie. 1973;55(2):205-11 PMID: 4578278
-
Intracellular proteinases in microorganisms.Curr Top Cell Regul. 1975;9:103-56 PMID: 1091413
-
Fructose-1,6-diphosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting and non fermenting yeasts.Arch Mikrobiol. 1971;76(2):132-8 PMID: 4324161
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Phosphopyruvate carboxylase from Baker's yeast. III. The mechanism of oxalocetate decarboxylation.J Biol Chem. 1963 Jun;238:1919-27 PMID: 14018314
-
Degradation of abnormal proteins in Escherichia coli (protein breakdown-protein structure-mistranslation-amino acid analogs-puromycin).Proc Natl Acad Sci U S A. 1972 Feb;69(2):422-6 PMID: 4551144
-
Glucose induced inactivation of malate dehydrogenase in intact yeast cells.Eur J Biochem. 1968 Jan;3(3):326-31 PMID: 5645528
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
