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PMID: 9469820 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The yeast transcription factor genes YAP1 and YAP2 are subject to differential control at the levels of both translation and mRNA stability.

Nucleic acids research ·Vol. 26 ·No. 5 ·1998-03-01 ·Pages 1150-9

Vilela C, Linz B, Rodrigues-Pousada C, McCarthy JE

Abstract

Two forms of post-transcriptional control direct differential expression of the Saccharomyces cerevisiae genes encoding the AP1-like transcription factors Yap1p and Yap2p. The mRNAs of these genes contain respectively one (YAP1 uORF) and two (YAP2 uORF1 and uORF2) upstream open reading frames. uORF-mediated modulation of post-termination events on the 5'-untranslated region (5'-UTR) directs differential control not only of translation but also of mRNA decay. Translational control is defined by two types of uORF function. The YAP1 -type uORF allows scanning 40S subunits to proceed via leaky scanning and re-initiation to the major ORF, whereas the YAP2 -type acts to block ribosomal scanning by promoting efficient termination. At the same time, the YAP2 uORFs define a new type of mRNA destabilizing element. Both post-termination ribosome scanning behaviour and mRNA decay are influenced by the coding sequence and mRNA context of the respective uORFs, including downstream elements. Our data indicate that release of post-termination ribosomes promotes largely upf -independent accelerated decay. It follows that translational termination on the 5'-UTR of a mature, non-aberrant yeast mRNA can trigger destabilization via a different pathway to that used to rid the cell of mRNAs containing premature stop codons. This route of control of non-aberrant mRNA decay influences the stress response in yeast. It is also potentially relevant to expression of the sizable number of eukaryotic mRNAs that are now recognized to contain uORFs.

MeSH Terms
Base Sequence DNA, Fungal/genetics DNA-Binding Proteins/genetics Drug Stability Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Molecular Sequence Data Open Reading Frames Protein Biosynthesis RNA Processing, Post-Transcriptional RNA, Fungal/genetics,metabolism RNA, Messenger/genetics,metabolism Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Transcription Factors/genetics
Chemicals
CAD1 protein, S cerevisiae DNA, Fungal DNA-Binding Proteins Fungal Proteins RNA, Fungal RNA, Messenger Saccharomyces cerevisiae Proteins Transcription Factors YAP1 protein, S cerevisiae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Vilela C
Posttranscriptional Control Group, Department of Biomolecular Sciences, University of Manchester Institute of Science and Technology (UMIST), PO Box 88, Manchester M60 1QD, UK.
Linz B
Rodrigues-Pousada C
McCarthy J E
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/26.5.1150
Published
1998-03-01
Pages
1150-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC147385
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/26.5.1150 PMC Full Text View journal details
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