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PMID: 1878996 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The SNQ3 gene of Saccharomyces cerevisiae confers hyper-resistance to several functionally unrelated chemicals.

Current genetics ·Vol. 19 ·No. 6 ·1991-06-00 ·Pages 429-33

Hertle K, Haase E, Brendel M

Abstract

A multi-copy plasmid containing the SNQ3 gene confers hyper-resistance to 4-nitroquinoline-N-oxide (4NQO), Trenimon, MNNG, cycloheximide, and to sulfometuron methyl in yeast transformants. Restriction analysis, subcloning, and DNA sequencing revealed an open reading frame of 1,950 bp on the SNQ3-containing insert DNA. Gene disruption and transplacement into chromosomal DNA yielded 4NQO-sensitive null mutants which were also more sensitive than the wild-type to Trenimon, cycloheximide, sulfometuron methyl, and MNNG. Hydropathic analysis showed that the SNQ3-encoded protein is most likely not membrane-bound, while the codon bias index points to low expression of the gene.

Related Genes
SNQ2 SNQ3
MeSH Terms
Amino Acid Sequence Antifungal Agents/pharmacology Base Sequence Cloning, Molecular Drug Resistance, Microbial Gene Expression Regulation, Fungal/drug effects Genes, Fungal Molecular Sequence Data Phenotype Saccharomyces cerevisiae/drug effects,genetics
Chemicals
Antifungal Agents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hertle K
Institut für Mikrobiologie, J.W. Goethe-Universität, Frankfurt am Main, Federal Republic of Germany.
Haase E
Brendel M
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Article Info
Journal
Current genetics
Abbr.
Curr Genet
ISSN
0172-8083
DOI
10.1007/BF00312733
Published
1991-06-00
Pages
429-33
Language
English
Region
United States
NLM ID
8004904
Subset
IM
Databases
GENBANK
S39723, S39727, S39777, S57532, S60919, S60920, X62724, X62725, X62800, X63268
External Links
PubMed source DOI: 10.1007/BF00312733 View journal details
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