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PMID: 2823103 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Saccharomyces cerevisiae positive regulatory gene PET111 encodes a mitochondrial protein that is translated from an mRNA with a long 5' leader.

Molecular and cellular biology ·Vol. 7 ·No. 8 ·1987-08-00 ·Pages 2728-34

Strick CA, Fox TD

Abstract

The yeast nuclear gene PET111 is required specifically for translation of the mitochondrion-coded mRNA for cytochrome c oxidase subunit II. We have determined the nucleotide sequence of a 3-kilobase segment of DNA that carries PET111. The sequence contains a single long open reading frame that predicts a basic protein of 718 amino acids. The PET111 gene product is a mitochondrial protein, since a hybrid protein which includes the amino-terminal 154 amino acids of PET111 fused to beta-galactosidase is specifically associated with mitochondria. PET111 is translated from a 2.9-kilobase mRNA which, interestingly, has an extended 5'-leader sequence containing four short open reading frames upstream of the long open reading frame. These open reading frames exhibit an interesting pattern of overlap with each other and with the PET111 reading frame.

MeSH Terms
Amino Acid Sequence Base Sequence DNA Restriction Enzymes Fungal Proteins/genetics Genes Genes, Fungal Genes, Regulator Mitochondria/metabolism Protein Biosynthesis Protein Sorting Signals/metabolism RNA, Messenger/genetics Saccharomyces cerevisiae/genetics,metabolism
Chemicals
Fungal Proteins Protein Sorting Signals RNA, Messenger DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Strick C A
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853.
Fox T D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.8.2728-2734.1987
Published
1987-08-00
Pages
2728-34
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367889
Subset
IM
Grants
NIGMS NIH HHS · GM07273 · United States
NIGMS NIH HHS · GM29362 · United States
NICHD NIH HHS · HD00515 · United States
Databases
GENBANK
M17143
External Links
PubMed source DOI: 10.1128/mcb.7.8.2728-2734.1987 PMC Full Text View journal details
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