Home LiteratureArticle Details
PMID: 9422604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and physical mapping of the daptomycin gene cluster from Streptomyces roseosporus.

Journal of bacteriology ·Vol. 180 ·No. 1 ·1998-01-00 ·Pages 143-51

Mchenney MA, Hosted TJ, Dehoff BS, Rosteck PR, Baltz RH

Abstract

The daptomycin biosynthetic gene cluster of Streptomyces roseosporus was analyzed by Tn5099 mutagenesis, molecular cloning, partial DNA sequencing, and insertional mutagenesis with cloned segments of DNA. The daptomycin biosynthetic gene cluster spans at least 50 kb and is located about 400 to 500 kb from one end of the approximately 7,100-kb linear chromosome. We identified two peptide synthetase coding regions interrupted by a 10- to 20-kb region that may encode other functions in lipopeptide biosynthesis.

MeSH Terms
Bacterial Proteins/genetics Chromosome Mapping/methods Cloning, Molecular Daptomycin/biosynthesis Genes, Bacterial/genetics Multigene Family/genetics Mutagenesis, Insertional Peptide Synthases/genetics Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Streptomyces/enzymology,genetics,metabolism
Chemicals
Bacterial Proteins CpsA protein, Streptomyces roseosporus cpsB protein, Streptomyces roseosporus Peptide Synthases Daptomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mchenney M A
Lilly Research Laboratories, A Division of Eli Lilly and Company, Indianapolis, Indiana 46285, USA.
Hosted T J
Dehoff B S
Rosteck P R
Baltz R H
References (31)
31 references, click to expand
  1. The Streptomyces lividans 66 chromosome contains a 1 MB deletogenic region flanked by two amplifiable regions.
    Mol Gen Genet. 1993 Nov;241(3-4):255-62 PMID: 8246878
  2. Deacylation of A21978C, an acidic lipopeptide antibiotic complex, by Actinoplanes utahensis.
    J Antibiot (Tokyo). 1988 Aug;41(8):1085-92 PMID: 3170344
  3. Physical map of the Streptomyces lividans 66 genome and comparison with that of the related strain Streptomyces coelicolor A3(2).
    J Bacteriol. 1993 Jun;175(11):3422-9 PMID: 8501047
  4. The multiple carrier model of nonribosomal peptide biosynthesis at modular multienzymatic templates.
    J Biol Chem. 1996 Jun 28;271(26):15428-35 PMID: 8663196
  5. Enzymatic and chemical modifications of lipopeptide antibiotic A21978C: the synthesis and evaluation of daptomycin (LY146032).
    J Antibiot (Tokyo). 1988 Aug;41(8):1093-105 PMID: 2844711
  6. Gene transfer and transposition mutagenesis in Streptomyces roseosporus: mapping of insertions that influence daptomycin or pigment production.
    Microbiology. 1996 Sep;142 ( Pt 9):2363-73 PMID: 8828204
  7. Amino acid activation and polymerization at modular multienzymes in nonribosomal peptide biosynthesis.
    Amino Acids. 1996 Sep;10(3):201-27 PMID: 24178536
  8. Rational design of peptide antibiotics by targeted replacement of bacterial and fungal domains.
    Science. 1995 Jul 7;269(5220):69-72 PMID: 7604280
  9. Engineered biosynthesis of peptide antibiotics.
    Biochem Pharmacol. 1996 Jul 26;52(2):177-86 PMID: 8694841
  10. Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus.
    J Bacteriol. 1997 Jan;179(1):180-6 PMID: 8981996
  11. A nonribosomal system of peptide biosynthesis.
    Eur J Biochem. 1996 Mar 1;236(2):335-51 PMID: 8612601
  12. Biosynthesis of acylpeptidolactones of the daptomycin type. A comparative analysis of peptide synthetases forming A21978C and A54145.
    Eur J Biochem. 1996 Dec 15;242(3):665-73 PMID: 9022695
  13. The chromosomal DNA of Streptomyces lividans 66 is linear.
    Mol Microbiol. 1993 Dec;10(5):923-33 PMID: 7934869
  14. Lipoteichoic acid as a target for antimicrobial action.
    Microb Drug Resist. 1996 Spring;2(1):85-9 PMID: 9158727
  15. A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.
    Mol Microbiol. 1996 Jul;21(1):77-96 PMID: 8843436
  16. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp.
    Gene. 1992 Jul 1;116(1):43-9 PMID: 1628843
  17. Hypertransposing derivatives of the streptomycete insertion sequence IS493.
    Gene. 1994 Sep 15;147(1):47-54 PMID: 8088548
  18. The unstable region of Streptomyces ambofaciens includes 210 kb terminal inverted repeats flanking the extremities of the linear chromosomal DNA.
    Mol Microbiol. 1996 Jan;19(2):261-71 PMID: 8825772
  19. Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus.
    J Bacteriol. 1991 Feb;173(3):1096-104 PMID: 1846854
  20. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  21. Delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase, the multienzyme integrating the four primary reactions in beta-lactam biosynthesis, as a model peptide synthetase.
    Biotechnology (N Y). 1993 Jul;11(7):807-10 PMID: 7763859
  22. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  23. Biochemical characterization of peptidyl carrier protein (PCP), the thiolation domain of multifunctional peptide synthetases.
    Chem Biol. 1996 Nov;3(11):913-21 PMID: 8939706
  24. A21978C, a complex of new acidic peptide antibiotics: isolation, chemistry, and mass spectral structure elucidation.
    J Antibiot (Tokyo). 1987 Jun;40(6):761-77 PMID: 3610833
  25. Inversions and deletions generated by a mini-gamma delta (Tn1000) transposon.
    J Bacteriol. 1994 Mar;176(5):1332-8 PMID: 8113172
  26. Nucleotide binding by multienzyme peptide synthetases.
    Eur J Biochem. 1994 Mar 1;220(2):535-42 PMID: 8125112
  27. Modular structure of genes encoding multifunctional peptide synthetases required for non-ribosomal peptide synthesis.
    FEMS Microbiol Lett. 1995 Jan 1;125(1):3-14 PMID: 7867917
  28. Genetic recombination in Streptomyces fradiae by protoplast fusion and cell regeneration.
    J Gen Microbiol. 1978 Jul;107(1):93-102 PMID: 731205
  29. A simple and effective electroporation apparatus.
    Biotechniques. 1990 Jan;8(1):28-30 PMID: 2182075
  30. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  31. Tn5099, a xylE promoter probe transposon for Streptomyces spp.
    J Bacteriol. 1991 Sep;173(17):5573-7 PMID: 1653213
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.180.1.143-151.1998
Published
1998-01-00
Pages
143-51
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC106860
Subset
IM
Databases
GENBANK
AF021262, AF021263
External Links
PubMed source DOI: 10.1128/JB.180.1.143-151.1998 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2