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PMID: 1846854 Published · ppublish English Journal Article

Transposition of Tn5096 and other IS493 derivatives in Streptomyces griseofuscus.

Journal of bacteriology ·Vol. 173 ·No. 3 ·1991-02-00 ·Pages 1096-104

Solenberg PJ, Baltz RH

Abstract

Tn5096 was constructed by inserting an apramycin resistance gene, aac(3)IV, into IS493 from Streptomyces lividans. By using conventional and pulsed-field gel electrophoresis, Tn5096 and related transposons were shown to insert into many different locations in the Streptomyces griseofuscus chromosome and in two linear plasmids. On insertion into the target site CANTg, 3 bp appeared to be duplicated. Independent transpositions were obtained by delivery of the transposon from a temperature-sensitive plasmid. The frequency of auxotrophy among cultures containing transpositions was about 0.2%.

MeSH Terms
Anti-Bacterial Agents/biosynthesis Base Sequence Cloning, Molecular DNA Transposable Elements Drug Resistance, Microbial/genetics Macrolides Molecular Sequence Data Nebramycin/analogs & derivatives,pharmacology Plasmids Restriction Mapping Spores, Bacterial/physiology Streptomyces/genetics,physiology Temperature
Chemicals
Anti-Bacterial Agents DNA Transposable Elements Macrolides Nebramycin lankacidins apramycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Solenberg P J
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Baltz R H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.3.1096-1104.1991
Published
1991-02-00
Pages
1096-104
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207229
Subset
IM
Databases
GENBANK
M63678, M63692, M63693, M63694, S70331, S70358, S70359, S70363, S70365, S70370
External Links
PubMed source DOI: 10.1128/jb.173.3.1096-1104.1991 PMC Full Text View journal details
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