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PMID: 1653213 Published · ppublish English Journal Article

Tn5099, a xylE promoter probe transposon for Streptomyces spp.

Journal of bacteriology ·Vol. 173 ·No. 17 ·1991-09-00 ·Pages 5573-7

Hahn DR, Solenberg PJ, Baltz RH

Abstract

Tn5099, a promoter probe transposon for Streptomyces spp., was constructed by inserting a promoterless xylE gene and a hygromycin resistance gene into IS493. Tn5099 transposed into different sites in the Streptomyces griseofuscus genome, and the xylE reporter gene was expressed in some of the transposition mutants. Strains containing Tn5099 insertions that gave regulated expression of the xylE gene were identified.

Related Genes
xylE
MeSH Terms
Amino Acid Sequence Base Sequence Catechol 2,3-Dioxygenase Cinnamates DNA Transposable Elements/genetics DNA, Bacterial Dioxygenases Drug Resistance, Microbial/genetics Electrophoresis Gene Expression Regulation Genes, Bacterial Hygromycin B/analogs & derivatives,pharmacology Molecular Sequence Data Mutation Oxygenases/genetics Promoter Regions, Genetic Restriction Mapping Streptomyces/genetics
Chemicals
Cinnamates DNA Transposable Elements DNA, Bacterial Hygromycin B hygromycin A Oxygenases Dioxygenases Catechol 2,3-Dioxygenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hahn D R
Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.
Solenberg P J
Baltz R H
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19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.17.5573-5577.1991
Published
1991-09-00
Pages
5573-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208275
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.17.5573-5577.1991 PMC Full Text View journal details
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