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PMID: 8843436 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.

Molecular microbiology ·Vol. 21 ·No. 1 ·1996-07-00 ·Pages 77-96

Redenbach M, Kieser HM, Denapaite D, Eichner A, Cullum J, Kinashi H, Hopwood DA

Abstract

A Supercos-1 library carrying chromosomal DNA of a plasmid-free derivative of Streptomyces coelicolor A3(2) was organized into an ordered encyclopaedia of overlapping clones by hybridization. The minimum set of overlapping clones representing the entire chromosome (with three short gaps) consists of 319 cosmids. The average insert size is 37.5 kb and the set of clones therefore divides the chromosome into 637 alternating unique and overlapping segments which have an average length of approx. 12.5 kb. More than 170 genes, gene clusters and other genetic markers were mapped to their specific segment by hybridization to the encyclopaedia. Genes could be cloned by direct transformation and complementation of S. coelicolor mutants with cosmids isolated from Escherichia coli, selecting for insertion into the chromosome by homologous recombination. As in other streptomycetes, the ends of the chromosome have long terminal inverted repeats.

MeSH Terms

Chromosome Mapping Cloning, Molecular Cosmids DNA, Bacterial/genetics,isolation & purification Escherichia coli/genetics Gene Library Genes, Bacterial Genetic Complementation Test Genetic Markers Molecular Sequence Data Multigene Family Mutation Recombination, Genetic Repetitive Sequences, Nucleic Acid Streptomyces/genetics Transformation, Genetic

Chemicals

DNA, Bacterial Genetic Markers

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Redenbach M

LB Genetik, Universität Kaiserslautern, Germany.

Kieser H M

Denapaite D

Eichner A

Cullum J

Kinashi H

Hopwood D A

Article Info

Journal

Molecular microbiology

Abbr.

Mol Microbiol

ISSN

0950-382X

DOI

10.1046/j.1365-2958.1996.6191336.x

Published

1996-07-00

Pages

77-96

Language

English

Region

England

NLM ID

8712028

Subset

Databases

GENBANK

AJ001493, AL009199, AL009204, AL020958, AL021409, AL021411, AL021529, AL021530, AL022268, AL022374, AL023496, AL023517, AL023702, AL023797, AL023861, AL023862, AL031013, AL031031, AL031035, AL031107, AL031124, AL031155, AL031182, AL031184, AL031225, AL031231, AL031232

External Links

PubMed source DOI: 10.1046/j.1365-2958.1996.6191336.x View journal details

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A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome.

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