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PMID: 9254693 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S. Review

Homing endonucleases: keeping the house in order.

Nucleic acids research ·Vol. 25 ·No. 17 ·1997-09-01 ·Pages 3379-88

Belfort M, Roberts RJ

Abstract

Homing endonucleases are rare-cutting enzymes encoded by introns and inteins. They have striking structural and functional properties that distinguish them from restriction enzymes. Nomenclature conventions analogous to those for restriction enzymes have been developed for the homing endonucleases. Recent progress in understanding the structure and function of the four families of homing enzymes is reviewed. Of particular interest are the first reported structures of homing endonucleases of the LAGLIDADG family. The exploitation of the homing enzymes in genome analysis and recombination research is also summarized. Finally, the evolution of homing endonucleases is considered, both at the structure-function level and in terms of their persistence in widely divergent biological systems.

MeSH Terms
Base Sequence Biological Evolution DNA, Recombinant Deoxyribonucleases/chemistry,metabolism Deoxyribonucleases, Type II Site-Specific/chemistry,metabolism Models, Molecular Molecular Sequence Data Saccharomyces cerevisiae Proteins Structure-Activity Relationship Substrate Specificity Terminology as Topic
Chemicals
DNA, Recombinant Saccharomyces cerevisiae Proteins Deoxyribonucleases SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Belfort M
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, PO Box 22002, Albany, New York 12201-2002, USA. marlene.belfort@wadsworth.org
Roberts R J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/25.17.3379
Email
marlene.belfort@wadsworth.org
Published
1997-09-01
Pages
3379-88
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146926
Subset
IM
Grants
NIGMS NIH HHS · GM39422 · United States
NIGMS NIH HHS · GM44844 · United States
NLM NIH HHS · LM04971 · United States
External Links
PubMed source DOI: 10.1093/nar/25.17.3379 PMC Full Text View journal details
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