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PMID: 8692957 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Double-strand break repair in the absence of RAD51 in yeast: a possible role for break-induced DNA replication.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 93 ·No. 14 ·1996-07-09 ·Pages 7131-6

Malkova A, Ivanov EL, Haber JE

Abstract

In wild-type diploid cells of Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) at the MAT locus can be efficiently repaired by gene conversion using the homologous chromosome sequences. Repair of the broken chromosome was nearly eliminated in rad52delta diploids; 99% lost the broken chromosome. However, in rad51delta diploids, the broken chromosomes were repaired approximately 35% of the time. None of these repair events were simple gene conversions or gene conversions with an associated crossover, instead, they created diploids homozygous for the MAT locus and all markers in the 100-kb region distal to the site of the DSB. In rad51delta diploids, the broken chromosome can apparently be inherited for several generations, as many of these repair events are found as sectored colonies, with one part being repaired and the other part being lost the broken chromosome. Similar events occur in about 2% of wild-type cells. We propose that a broken chromosome end can invade a homologous template in the absence of RAD51 and initiate DNA replication that may extend to the telomere, 100 or more kb away. Such break-induced replication appears to be similar to recombination-initiated replication in bacteria.

MeSH Terms
Chromosomes, Fungal DNA Damage DNA Repair DNA Replication DNA, Fungal/biosynthesis,genetics DNA-Binding Proteins/genetics,metabolism Diploidy Fungal Proteins/metabolism Gene Conversion Homozygote Mating Factor Peptides/genetics Rad51 Recombinase Recombination, Genetic Saccharomyces cerevisiae/genetics,metabolism Saccharomyces cerevisiae Proteins Telomere Templates, Genetic
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Peptides Saccharomyces cerevisiae Proteins Mating Factor RAD51 protein, S cerevisiae Rad51 Recombinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Malkova A
Rosenstiel Center and Department of Biology, Brandeis University, Waltham, MA 02254-9110, USA.
Ivanov E L
Haber J E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.93.14.7131
Published
1996-07-09
Pages
7131-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC38948
Subset
IM
Grants
NIGMS NIH HHS · GM20056 · United States
External Links
PubMed source DOI: 10.1073/pnas.93.14.7131 PMC Full Text View journal details
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