Home LiteratureArticle Details
PMID: 2159153 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Characterization of the restriction site of a prokaryotic intron-encoded endonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 9 ·1990-05-00 ·Pages 3574-8

Chu FK, Maley G, Pedersen-Lane J, Wang AM, Maley F

Abstract

The 1016-base-pair (bp) intron in the T4 bacteriophage thymidylate synthase gene (td) contains a 735-bp open reading frame that encodes a protein product with endonucleolytic activity. The endonuclease shows specificity for the intronless form of the td gene. Highly purified endonuclease cleaves the DNA of the intronless form of the td gene in vitro at 24 bp upstream of the exon 1-exon 2 junction, generating a 2-base staggered cut with 3'-hydroxyl overhangs. Although the endonuclease cleaves in exon 1, it requires some exon 2 sequence for recognition. The maximum recognition sequence lies in an 87-bp stretch, from 52 bp upstream to 35 bp downstream of the cleavage site, ending at 11 bp into exon 2. The td intron endonuclease appears involved in the conversion of the intronless form of td to intron-containing td gene in the T-even phages. A role for intron mobility is discussed.

MeSH Terms
Base Sequence Endonucleases/genetics,metabolism Escherichia coli/genetics Exons Gene Conversion Genes, Viral Introns Molecular Sequence Data Restriction Mapping Substrate Specificity T-Phages/enzymology,genetics Thymidylate Synthase/genetics Viral Structural Proteins/genetics
Chemicals
Viral Structural Proteins Thymidylate Synthase Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chu F K
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Maley G
Pedersen-Lane J
Wang A M
Maley F
References (36)
36 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Cloning and expression of an intron-deleted phage T4 td gene.
    J Biol Chem. 1986 Oct 15;261(29):13446-50 PMID: 3020032
  3. Critical sequences within mitochondrial introns: pleiotropic mRNA maturase and cis-dominant signals of the box intron controlling reductase and oxidase.
    Cell. 1982 Apr;28(4):721-32 PMID: 6284370
  4. Making ends meet: a model for RNA splicing in fungal mitochondria.
    Nature. 1982 Dec 23;300(5894):719-24 PMID: 6757759
  5. Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.
    Biochimie. 1982 Oct;64(10):867-81 PMID: 6817818
  6. Putative introns in tRNA genes of prokaryotes.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3309-12 PMID: 6304706
  7. The use of pKc30 and its derivatives for controlled expression of genes.
    Methods Enzymol. 1983;101:123-38 PMID: 6310317
  8. An mRNA maturase is encoded by the first intron of the mitochondrial gene for the subunit I of cytochrome oxidase in S. cerevisiae.
    Cell. 1983 Dec;35(3 Pt 2):733-42 PMID: 6317200
  9. Intervening sequence in the thymidylate synthase gene of bacteriophage T4.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3049-53 PMID: 6328492
  10. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  11. In vitro expression of the intron-containing gene for T4 phage thymidylate synthase.
    J Biol Chem. 1985 Sep 5;260(19):10680-8 PMID: 3897223
  12. Universal code equivalent of a yeast mitochondrial intron reading frame is expressed into E. coli as a specific double strand endonuclease.
    Cell. 1986 Feb 28;44(4):521-33 PMID: 3004738
  13. Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript.
    Cell. 1986 Apr 25;45(2):157-66 PMID: 3698096
  14. RNA splicing and in vivo expression of the intron-containing td gene of bacteriophage T4.
    Gene. 1986;41(1):93-102 PMID: 2422090
  15. Biological catalysis by RNA.
    Annu Rev Biochem. 1986;55:599-629 PMID: 2427016
  16. Multiple self-splicing introns in bacteriophage T4: evidence from autocatalytic GTP labeling of RNA in vitro.
    Cell. 1986 Oct 10;47(1):81-7 PMID: 3757035
  17. The bacteriophage T4 gene for the small subunit of ribonucleotide reductase contains an intron.
    EMBO J. 1986 Aug;5(8):2031-6 PMID: 3530746
  18. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  19. Nucleotide sequence and primary structures of gene products coded for by the T4 genome between map positions 48.266 kb and 39.166 kb.
    Nucleic Acids Res. 1987 Apr 24;15(8):3632-3 PMID: 3575111
  20. Mechanism and requirements of in vitro RNA splicing of the primary transcript from the T4 bacteriophage thymidylate synthase gene.
    Biochemistry. 1987 Jun 2;26(11):3050-7 PMID: 3038175
  21. Interrupted thymidylate synthase gene of bacteriophages T2 and T6 and other potential self-splicing introns in the T-even bacteriophages.
    J Bacteriol. 1987 Sep;169(9):4368-75 PMID: 2442142
  22. Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
    Gene. 1987;56(1):125-35 PMID: 3315856
  23. RNA splicing in the T-even bacteriophage.
    FASEB J. 1988 Mar 1;2(3):216-23 PMID: 3280375
  24. Structures involved in Tetrahymena rRNA self-splicing and RNA enzyme activity.
    Cold Spring Harb Symp Quant Biol. 1987;52:147-57 PMID: 3454258
  25. A family of autocatalytic group I introns in bacteriophage T4.
    Cold Spring Harb Symp Quant Biol. 1987;52:193-200 PMID: 2841063
  26. Recognition and cleavage site of the intron-encoded omega transposase.
    Proc Natl Acad Sci U S A. 1988 Aug;85(16):6022-6 PMID: 2842757
  27. A latent intron-encoded maturase is also an endonuclease needed for intron mobility.
    Cell. 1989 Feb 10;56(3):421-30 PMID: 2536592
  28. Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria.
    Cell. 1989 Feb 10;56(3):431-41 PMID: 2536593
  29. A mobile group I intron in the nuclear rDNA of Physarum polycephalum.
    Cell. 1989 Feb 10;56(3):443-54 PMID: 2536594
  30. Intron mobility in the T-even phages: high frequency inheritance of group I introns promoted by intron open reading frames.
    Cell. 1989 Feb 10;56(3):455-65 PMID: 2644046
  31. Evidence that the intron open reading frame of the phage T4 td gene encodes a specific endonuclease.
    J Biol Chem. 1989 Jun 25;264(18):10343-6 PMID: 2543665
  32. Exon shuffling by recombination between self-splicing introns of bacteriophage T4.
    Nature. 1989 Aug 17;340(6234):575-6 PMID: 2770862
  33. A site-specific endonuclease and co-conversion of flanking exons associated with the mobile td intron of phage T4.
    Gene. 1989 Oct 15;82(1):119-26 PMID: 2555262
  34. Mobile introns and intron-encoded proteins.
    Science. 1989 Dec 1;246(4934):1106-9 PMID: 2479980
  35. In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.
    Cell. 1981 Dec;27(3 Pt 2):487-96 PMID: 6101203
  36. Sequence of introns and flanking exons in wild-type and box3 mutants of cytochrome b reveals an interlaced splicing protein coded by an intron.
    Cell. 1980 Nov;22(2 Pt 2):333-48 PMID: 7004642
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.9.3574
Published
1990-05-00
Pages
3574-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53944
Subset
IM
Grants
NCI NIH HHS · CA44355 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.9.3574 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2