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PMID: 1848651 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new specific DNA endonuclease activity in yeast mitochondria.

Molecular & general genetics : MGG ·Vol. 225 ·No. 2 ·1991-02-00 ·Pages 340-1

Sargueil B, Delahodde A, Hatat D, Tian GL, Lazowska J, Jacq C

Abstract

Two group I intron-encoded proteins from the yeast mitochondrial genome have already been shown to have a specific DNA endonuclease activity. This activity mediates intron insertion by cleaving the DNA sequence corresponding to the splice junction of an intronless strain. We have discovered in mitochondrial extracts from the yeast strain 777-3A a new DNA endonuclease activity which cleaves the fused exon A3-exon A4 junction sequence of the CO XI gene.

Related Genes
COXI
MeSH Terms
Base Sequence Blotting, Southern DNA Restriction Enzymes/genetics,metabolism Deoxyribonucleases, Type II Site-Specific Endodeoxyribonucleases/genetics,metabolism Exons Genes, Fungal Introns Mitochondria/enzymology Molecular Sequence Data RNA Splicing Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins
Chemicals
Saccharomyces cerevisiae Proteins Endodeoxyribonucleases AI3 protein, S cerevisiae DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Sargueil B
Laboratoire de Génétique Moléculaire CNRS UA 1302, Paris, France.
Delahodde A
Hatat D
Tian G L
Lazowska J
Jacq C
References (9)
9 references, click to expand
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  2. Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria.
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  3. Mobile introns and intron-encoded proteins.
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  4. Group I introns as mobile genetic elements: facts and mechanistic speculations--a review.
    Gene. 1989 Oct 15;82(1):91-114 PMID: 2555264
  5. In vivo and in vitro analyses of an intron-encoded DNA endonuclease from yeast mitochondria. Recognition site by site-directed mutagenesis.
    Nucleic Acids Res. 1990 Oct 11;18(19):5659-65 PMID: 2216759
  6. Universal code equivalent of a yeast mitochondrial intron reading frame is expressed into E. coli as a specific double strand endonuclease.
    Cell. 1986 Feb 28;44(4):521-33 PMID: 3004738
  7. Infectious introns.
    Cell. 1989 Feb 10;56(3):323-6 PMID: 2536590
  8. A latent intron-encoded maturase is also an endonuclease needed for intron mobility.
    Cell. 1989 Feb 10;56(3):421-30 PMID: 2536592
  9. Bacteriophage introns: parasites within parasites?
    Trends Genet. 1989 Jul;5(7):209-13 PMID: 2675424
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00269867
Published
1991-02-00
Pages
340-1
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00269867
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