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PMID: 9234700 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Isolation and characterization of a novel epithelium-specific transcription factor, ESE-1, a member of the ets family.

Molecular and cellular biology ·Vol. 17 ·No. 8 ·1997-08-00 ·Pages 4419-33

Oettgen P, Alani RM, Barcinski MA, Brown L, Akbarali Y, Boltax J, Kunsch C, Munger K, Libermann TA

Abstract

We report here the isolation of a novel, highly tissue-restricted member of the ets transcription factor/oncogene family, ESE-1 (for epithelium-specific Ets), which has features distinct from those of any other ets-related factor. ESE-1 contains two putative DNA binding domains: an ETS domain, which is unique in that the 5' half shows relatively weak homology to known ets factors, and an A/T hook domain, found in HMG proteins and various other nuclear factors. In contrast to any known ets factors, ESE-1 is expressed exclusively in epithelial cells. ESE-1 expression is induced during terminal differentiation of the epidermis and in a primary human keratinocyte differentiation system. The keratinocyte terminal differentiation marker gene, SPRR2A, is a putative target for ESE-1, since SPRR2A expression during keratinocyte differentiation correlates with induction of ESE-1 expression, and ESE-1 binds with high affinity to and transactivates the ets binding site in the SPRR2A promoter. ESE-1 also binds to and transactivates the enhancer of the Endo A gene, a potential target for ESE-1 in simple epithelia. Due to the important role that other ets factors play in cellular differentiation, ESE-1 is expected to be a critical regulator of epithelial cell differentiation.

MeSH Terms
Alternative Splicing Amino Acid Sequence Animals Base Sequence Cell Differentiation Cells, Cultured Cloning, Molecular Cornified Envelope Proline-Rich Proteins DNA-Binding Proteins/genetics Epidermal Cells Epithelial Cells Epithelium/chemistry Gene Expression Regulation, Developmental High Mobility Group Proteins/genetics Humans Keratinocytes/chemistry,cytology Keratins/genetics Membrane Proteins/genetics Mice Molecular Sequence Data Nuclear Proteins Organ Specificity Protein Precursors/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins c-ets RNA, Messenger/analysis Sequence Analysis, DNA Sequence Homology, Amino Acid Trans-Activators/chemistry,genetics Transcription Factors/genetics
Chemicals
Cornified Envelope Proline-Rich Proteins DNA-Binding Proteins ELF1 protein, human ELF3 protein, human Elf1 protein, mouse High Mobility Group Proteins Membrane Proteins Nuclear Proteins Protein Precursors Proto-Oncogene Proteins Proto-Oncogene Proteins c-ets RNA, Messenger SPRR2A protein, human Sprr2a1 protein, mouse Trans-Activators Transcription Factors Keratins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Oettgen P
Division of Immunology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts 02215, USA.
Alani R M
Barcinski M A
Brown L
Akbarali Y
Boltax J
Kunsch C
Munger K
Libermann T A
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.17.8.4419
Published
1997-08-00
Pages
4419-33
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC232296
Subset
IM
Grants
NIAID NIH HHS · AI/CA33211-05 · United States
NCI NIH HHS · KO8CA 71429-01 · United States
Databases
GENBANK
U73843, U73844
External Links
PubMed source DOI: 10.1128/MCB.17.8.4419 PMC Full Text View journal details
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