Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma.

The EMBO journal ·Vol. 7 ·No. 10 ·1988-10-00 ·Pages 3181-7

Schlegel R, Phelps WC, Zhang YL, Barbosa M

Abstract

Keratinocytes electroporated with human papillomavirus (HPV) DNA (HPV-6, 11, 16 and 18) exhibited an increased cellular proliferation which was quantitated as microcolony and macrocolony formation. However, only macrocolonies induced by HPV-16 or HPV-18 DNA (the two viral types most commonly found in human cervical carcinomas) gave rise to proliferating, poorly-stratified colonies when grown in the presence of serum and calcium. Hydrocortisone increased the frequency of these differentiation-resistant colonies, and studies showed that they were immortalized, contained one copy of viral DNA per cell, expressed three discrete species of viral RNA and synthesized the viral E7 protein. HPV-induced cellular proliferation and altered differentiation are therefore separable events and may represent the activity of different viral genes.

MeSH Terms

Biological Assay Blotting, Southern Calcium/pharmacology Cell Differentiation/drug effects Cell Division Cell Transformation, Viral DNA, Viral/genetics Epidermal Cells Female Humans In Vitro Techniques Keratins Male Papillomaviridae/genetics,pathogenicity Uterine Cervical Neoplasms/microbiology Viral Proteins/metabolism

Chemicals

DNA, Viral Viral Proteins Keratins Calcium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schlegel R

National Cancer Institute, Bethesda, MD 20892.

Phelps W C

Zhang Y L

Barbosa M

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36 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1988-10-00

Pages

3181-7

Language

English

Region

England

NLM ID

8208664

PMCID

PMC454709

Subset

External Links

PubMed source PMC Full Text View journal details

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Quantitative keratinocyte assay detects two biological activities of human papillomavirus DNA and identifies viral types associated with cervical carcinoma.

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