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PMID: 9098074 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Novel Vibrio cholerae O139 genes involved in lipopolysaccharide biosynthesis.

Journal of bacteriology ·Vol. 179 ·No. 8 ·1997-04-00 ·Pages 2740-7

Stroeher UH, Parasivam G, Dredge BK, Manning PA

Abstract

The sequence of part of the rfb region of Vibrio cholerae serogroup O139 and the physical map of a 35-kb region of the O139 chromosome have been determined. The O139 rfb region presented contains a number of open reading frames which show similarities to other rfb and capsular biosynthesis genes found in members of the Enterobacteriaceae family and in V. cholerae O1. The cloned and sequenced region can complement the defects in O139 antigen biosynthesis in transposon insertions within the O139 rfb cluster. Linkage is demonstrated among IS1358 of V. cholerae O139, the rfb region, and the recently reported otnA and otnB genes (E. M. Bik, A. E. Bunschoten, R. D. Gouw, and F. R. Mooi, EMBO J. 14:209-216, 1995). In addition, the whole of this region has been linked to the rfaD gene. Furthermore, determination of the sequence flanking IS1358 has revealed homology to other rfb-like genes. The exact site of insertion with respect to rfaD is defined for the novel DNAs of both the Bengal and the Argentinian O139 isolates.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/biosynthesis Argentina Bacterial Capsules/genetics Bacterial Proteins/genetics Base Sequence Carbohydrate Epimerases/genetics Cloning, Molecular DNA Transposable Elements/genetics Genes, Bacterial/genetics Genetic Complementation Test India Lipopolysaccharides/biosynthesis Molecular Sequence Data Open Reading Frames/genetics Restriction Mapping Sequence Analysis, DNA Sequence Homology, Nucleic Acid Vibrio cholerae/genetics,immunology
Chemicals
Antigens, Bacterial Bacterial Proteins DNA Transposable Elements Lipopolysaccharides rfb protein, Bacteria ADP-L-glycero-D-mannoheptose-6-epimerase Carbohydrate Epimerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Stroeher U H
Department of Microbiology and Immunology, University of Adelaide, South Australia.
Parasivam G
Dredge B K
Manning P A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.8.2740-2747.1997
Published
1997-04-00
Pages
2740-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC179025
Subset
IM
Databases
GENBANK
Y07786
External Links
PubMed source DOI: 10.1128/jb.179.8.2740-2747.1997 PMC Full Text View journal details
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