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PMID: 9079898 Published · ppublish English Journal Article

Identification of additional genes required for O-antigen biosynthesis in Vibrio cholerae O1.

Journal of bacteriology ·Vol. 179 ·No. 7 ·1997-04-00 ·Pages 2147-53

Fallarino A, Mavrangelos C, Stroeher UH, Manning PA

Abstract

The cloning and expression of the genes encoding the Vibrio cholerae O1 lipopolysaccharide O antigen in a heterologous host have been described previously (P. A. Manning, M. W. Heuzenroeder, J. Yeadon, D. I. Leavesley, P. R. Reeves, and D. Rowley, Infect. Immun. 53:272-277, 1986). It was thus assumed that all the genes required for O-antigen expression were located on a 20-kb SacI restriction fragment. We present evidence for a number of other as yet undescribed genes that are essential for O-antigen biosynthesis in V. cholerae O1 and that these genes are somehow complemented in Escherichia coli K-12. The two genes termed Vibrio cholerae rfbV and rfbU are transcribed in the opposite orientation from the rest of the rfb operon, whereas the galE dehydratase and rfbP (Salmonella enterica) homologs, designated ORF35x7 and rfbW, respectively, are transcribed in the same orientation. The evidence presented here, using chromosomal insertion mutants, clearly shows that the three genes now designated rfbV, rfbU, and rfbW appear to be accessory rfb genes and are essential for O-antigen biosynthesis in V. cholerae but that ORF35x7 is not.

MeSH Terms
Amino Acid Sequence Bacterial Capsules/metabolism Bacterial Proteins/genetics Genes, Bacterial Genetic Complementation Test Molecular Sequence Data O Antigens/biosynthesis Operon Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Vibrio cholerae/genetics
Chemicals
Bacterial Proteins O Antigens RfbU protein, Vibrio cholerae RfbV protein, Vibrio cholerae RfbW protein, Vibrio cholerae
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fallarino A
Department of Microbiology and Immunology, The University of Adelaide, South Australia.
Mavrangelos C
Stroeher U H
Manning P A
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.179.7.2147-2153.1997
Published
1997-04-00
Pages
2147-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC178949
Subset
IM
Databases
GENBANK
Y07788
External Links
PubMed source DOI: 10.1128/jb.179.7.2147-2153.1997 PMC Full Text View journal details
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