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PMID: 2793832 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and sequence of the gene for abequose synthase, which confers antigenic specificity on group B salmonellae: homology with galactose epimerase.

Journal of bacteriology ·Vol. 171 ·No. 10 ·1989-10-00 ·Pages 5687-93

Wyk P, Reeves P

Abstract

The O antigen of Salmonella group B strains contains the sugar abequose, whereas those from group A and D strains contain paratose or tyvelose in its place. This is the essential difference between these Salmonella groups. Only the final step in the biosynthesis of abequose differs from that of paratose, and the abequose confers on group B strains their specific O4 antigen. The gene, rfbJ, encoding the enzyme abequose synthase for this last specific step has been cloned, identified, and sequenced and has been shown to function in group A and D strains to make them O4+. This one gene thus differentiates group B from group A or group D salmonellae. The enzyme abequose synthase appears to be related to galactose epimerase, and the significance of this is discussed. The rfbJ gene and adjacent DNA is of much lower G+C content than is usual for salmonellae, indicating that the region did not originate in a salmonella but was transferred from outside.

MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Base Composition Base Sequence Carbohydrate Epimerases/genetics DNA Mutational Analysis Genes, Bacterial Hexoses Molecular Sequence Data Polysaccharides, Bacterial/genetics Salmonella/genetics,immunology UDPglucose 4-Epimerase
Chemicals
Antigens, Bacterial Hexoses Polysaccharides, Bacterial abequose Carbohydrate Epimerases abequose synthase UDPglucose 4-Epimerase galactose epimerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wyk P
Department of Microbiology and Immunology, University of Adelaide, Australia.
Reeves P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.10.5687-5693.1989
Published
1989-10-00
Pages
5687-93
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210415
Subset
IM
Databases
GENBANK
M29713
External Links
PubMed source DOI: 10.1128/jb.171.10.5687-5693.1989 PMC Full Text View journal details
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