Home LiteratureArticle Details
PMID: 1707412 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning, characterization, and nucleotide sequence of the rfc gene, which encodes an O-antigen polymerase of Salmonella typhimurium.

Journal of bacteriology ·Vol. 173 ·No. 8 ·1991-04-00 ·Pages 2521-9

Collins LV, Hackett J

Abstract

The rfc gene of Salmonella typhimurium was located in a 1.75-kb HindIII fragment and restored wild-type lipopolysaccharide synthesis ability to both an older rfc point mutant and new rfc::IS10 mutants. DNA sequencing of the HindIII fragment revealed an open reading frame which could encode a protein of 407 amino acids with an Mr of 47,472 and also revealed potential translation signals. Modulator codons accounted for 12.5% of the total codon content, providing a possible explanation for the nondetectability of the protein in subcellular systems. Secondary structure analysis suggested the presence of transmembrane beta-sheet structures, implying a possible role for the protein in translocation of hydrophilic O-antigen-containing materials. Salmonella strains of groups A, B, and D1 contained rfc-homologous DNA, but strains of groups C1, C2, C3, D2, and E2 did not.

Related Genes
rfc
MeSH Terms
Amino Acid Sequence Antigens, Bacterial/genetics Base Sequence Cloning, Molecular Codon Electrophoresis, Polyacrylamide Gel Genes, Bacterial Hexosyltransferases/genetics Molecular Sequence Data Mutagenesis, Insertional O Antigens Open Reading Frames Restriction Mapping Salmonella typhimurium/genetics
Chemicals
Antigens, Bacterial Codon O Antigens Hexosyltransferases O-antigen polymerase, Salmonella
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collins L V
Department of Microbiology, University of Adelaide, South Australia.
Hackett J
References (49)
49 references, click to expand
  1. Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.
    Mol Gen Genet. 1981;184(3):430-3 PMID: 6278256
  2. Positive selection for loss of tetracycline resistance.
    J Bacteriol. 1980 Aug;143(2):926-33 PMID: 6259126
  3. The smooth lipopolysaccharide character of 1,4,(5),12 and 1,9,12 transductants formed as hybrids between groups B and D of salmonella.
    Eur J Biochem. 1971 Oct 26;22(4):500-5 PMID: 4942844
  4. Mechanism of epsilon-15 conversion studies with a bacterial mutant.
    J Mol Biol. 1967 Dec 28;30(3):445-55 PMID: 4970573
  5. Structure of cell wall lipopolysaccharide from Salmonella typhimurium. Structure of lipopolysaccharide from a Semirough mutant.
    Eur J Biochem. 1970 Jul;15(1):63-71 PMID: 4923159
  6. Genetic determination of the O antigens of Salmonella groups B (4,5,12) and C1(6,7).
    J Bacteriol. 1966 Mar;91(3):1115-25 PMID: 5326095
  7. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  8. Inheritance of the O antigens of Salmonella groups B and D.
    J Gen Microbiol. 1965 Oct;41(1):57-65 PMID: 5883698
  9. Compilation and analysis of Escherichia coli promoter DNA sequences.
    Nucleic Acids Res. 1983 Apr 25;11(8):2237-55 PMID: 6344016
  10. Translational initiation in prokaryotes.
    Annu Rev Microbiol. 1981;35:365-403 PMID: 6170248
  11. Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2.
    Eur J Biochem. 1980;107(1):145-53 PMID: 6156828
  12. Association of adhesive, invasive, and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plasmids.
    Infect Immun. 1982 Nov;38(2):476-86 PMID: 6128304
  13. A small cosmid for efficient cloning of large DNA fragments.
    Gene. 1980 Nov;11(3-4):291-8 PMID: 6260575
  14. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  15. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  16. Versatile low-copy-number plasmid vectors for cloning in Escherichia coli.
    Gene. 1982 Jun;18(3):335-41 PMID: 6290337
  17. Codon usage and transfer RNA contents: organism-specific codon-choice patterns in reference to the isoacceptor contents.
    Cold Spring Harb Symp Quant Biol. 1983;47 Pt 2:1087-97 PMID: 6345068
  18. Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
    Gene. 1982 Jun;18(3):199-209 PMID: 6751939
  19. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  20. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  21. Cell-free synthesis of proteins coding for mobilisation functions of ColE1 and transposition functions of Tn3.
    Gene. 1979 May;6(1):29-42 PMID: 383577
  22. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  23. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics.
    J Mol Biol. 1977 Oct 15;116(1):125-59 PMID: 338917
  24. The transposon Tn1 as a probe for studying ColE1 structure and function.
    Mol Gen Genet. 1977 Mar 7;151(2):151-60 PMID: 327263
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Genetic determination of lipopolysaccharide: locus of O-specific unit polymerase in group E of salmonella.
    Zentralbl Bakteriol Orig A. 1979 Apr;243(2-3):355-62 PMID: 452771
  27. Prediction of protein conformation.
    Biochemistry. 1974 Jan 15;13(2):222-45 PMID: 4358940
  28. In vitro synthesis of protein in microbial systems.
    Annu Rev Genet. 1973;7:267-87 PMID: 4593305
  29. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  30. Effect of the quality of the lipopolysaccharide on mouse virulence of Salmonella enteritidis.
    Infect Immun. 1975 Oct;12(4):828-32 PMID: 53202
  31. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
  32. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping.
    J Gen Microbiol. 1972 May;70(3):527-54 PMID: 4556257
  33. SEMIROUGH STRAINS OF SALMONELLA.
    Proc Natl Acad Sci U S A. 1965 Jan;53:147-53 PMID: 14283192
  34. ROUGH MUTANTS OF SALMONELLA TYPHIMURIUM. I. GENETICS.
    Nature. 1964 Mar 28;201:1298-9 PMID: 14151409
  35. Galactose-sensitive mutants of Salmonella. II. Bacteriolysis induced by galactose.
    Biochim Biophys Acta. 1961 Apr 15;48:470-83 PMID: 13702505
  36. Molecular cloning and analysis of the incompatibility and partition functions of the virulence plasmid of Salmonella typhimurium.
    Microb Pathog. 1989 Aug;7(2):85-99 PMID: 2556621
  37. IS10 transposition is regulated by DNA adenine methylation.
    Cell. 1985 Nov;43(1):117-30 PMID: 3000598
  38. Tn1721 derivatives for transposon mutagenesis, restriction mapping and nucleotide sequence analysis.
    Gene. 1986;41(2-3):145-52 PMID: 3011592
  39. Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae.
    J Bacteriol. 1989 Oct;171(10):5694-701 PMID: 2793833
  40. Construction of defined galE mutants of Salmonella for use as vaccines.
    J Infect Dis. 1987 Jul;156(1):167-74 PMID: 3036954
  41. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
    Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767-71 PMID: 3474623
  42. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  43. Use of lambda vehicles to isolate ompC-lacZ gene fusions in Salmonella typhimurium LT2.
    Mol Gen Genet. 1987 Oct;209(3):607-11 PMID: 2828886
  44. Complete physical map of the rfb gene cluster encoding biosynthetic enzymes for the O antigen of Salmonella typhimurium LT2.
    J Bacteriol. 1988 Jan;170(1):98-102 PMID: 2447067
  45. New Tn10 derivatives for transposon mutagenesis and for construction of lacZ operon fusions by transposition.
    Gene. 1984 Dec;32(3):369-79 PMID: 6099322
  46. Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid.
    J Bacteriol. 1984 Sep;159(3):1034-9 PMID: 6384177
  47. Membrane protein folding and oligomerization: the two-stage model.
    Biochemistry. 1990 May 1;29(17):4031-7 PMID: 1694455
  48. Shigella sonnei plasmids: evidence that a large plasmid is necessary for virulence.
    Infect Immun. 1981 Oct;34(1):75-83 PMID: 6271687
  49. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.8.2521-2529.1991
Published
1991-04-00
Pages
2521-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC207816
Subset
IM
Databases
GENBANK
M55488, M55489, M55490, M55491, M55492, M55493, M55494, M60066, M93063, M93064
External Links
PubMed source DOI: 10.1128/jb.173.8.2521-2529.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2