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PMID: 6278256 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.

Molecular & general genetics : MGG ·Vol. 184 ·No. 3 ·1981-00-00 ·Pages 430-3

Morona R, Reeves P

Abstract

We have cloned the tolC gene of E. coli K-12 into pSF2124 by using transposon Tn10 as the marker to first isolate the relevant DNA fragment. The gene is on a 10.5 kb EcoRI fragment, and Tn5 insertion mutagenesis locates the gene near one end of this EcoRI fragment. An EcoRI-PstI fragment has been subcloned into pBR322 to facilitate further analysis of the gene.

MeSH Terms

Cloning, Molecular Coliphages/genetics DNA Restriction Enzymes DNA Transposable Elements Escherichia coli/genetics Genes Mutation Plasmids Species Specificity

Chemicals

DNA Transposable Elements DNA Restriction Enzymes

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Morona R

Reeves P

References (19)

19 references, click to expand

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Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00352517

Published

1981-00-00

Pages

430-3

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00352517

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Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn10.

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