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PMID: 9032326 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Episodic evolution mediates interspecies transfer of a murine coronavirus.

Journal of virology ·Vol. 71 ·No. 3 ·1997-03-00 ·Pages 1946-55

Baric RS, Yount B, Hensley L, Peel SA, Chen W

Abstract

Molecular mechanisms permitting the establishment and dissemination of a virus within a newly adopted host species are poorly understood. Mouse hepatitis virus (MHV) strains (MHV-A59, MHV-JHM, and MHV-A59/MHV-JHM) were passaged in mixed cultures containing progressively increasing concentrations of nonpermissive Syrian baby hamster kidney (BHK) cells and decreasing concentrations of permissive murine DBT cells. From MHV-A59/MHV-JHM mixed infection, variant viruses (MHV-H1 and MHV-H2) which replicated efficiently in BHK cells were isolated. Under identical treatment conditions, the parental MHV-A59 or MHV-JHM strains failed to produce infectious virus or transcribe detectable levels of viral RNA or protein. The MHV-H isolates were polytrophic, replicating efficiently in normally nonpermissive Syrian hamster smooth muscle (DDT-1), Chinese hamster ovary (CHO), human adenocarcinoma (HRT), primate kidney (Vero), and murine 17Cl-1 cell lines. Little if any virus replication was detected in feline kidney (CRFK) and porcine testicular (ST) cell lines. The variant virus, MHV-H2, transcribed seven mRNAs equivalent in relative abundance and size to those synthesized by the parental virus strains. MHV-H2 was an RNA recombinant virus containing a crossover site in the S glycoprotein gene. At the molecular level, episodic evolution and positive Darwinian natural selection were apparent within the MHV-H2 S and HE glycoprotein genes. These findings differ from the hypothesis that neutral changes are the predominant feature of molecular evolution and argue that changing ecologies actuate episodic evolution in the MHV spike glycoprotein genes that govern interspecies transfer and spread into alternative hosts.

MeSH Terms
Animals Biological Evolution CHO Cells Cats Cell Line Chlorocebus aethiops Cricetinae Genome, Viral Humans Mesocricetus Mice Murine hepatitis virus/genetics,isolation & purification RNA, Viral/biosynthesis Rats Swine Transcription, Genetic Tumor Cells, Cultured Vero Cells
Chemicals
RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baric R S
Department of Epidemiology, University of North Carolina at Chapel Hill, 27599-7400, USA.
Yount B
Hensley L
Peel S A
Chen W
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.71.3.1946-1955.1997
Published
1997-03-00
Pages
1946-55
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC191277
Subset
IM
Grants
PHS HHS · 5 T32 A107151-16 · United States
NIAID NIH HHS · AI23946 · United States
External Links
PubMed source DOI: 10.1128/JVI.71.3.1946-1955.1997 PMC Full Text View journal details
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