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PMID: 2002545 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Selection of genetic variants of simian immunodeficiency virus in persistently infected rhesus monkeys.

Journal of virology ·Vol. 65 ·No. 4 ·1991-04-00 ·Pages 1843-54

Burns DP, Desrosiers RC

Abstract

Genetic and antigenic variation may be one means by which lentiviruses that cause AIDS avoid elimination by host immune responses. Genetic variation in the envelope gene (env) was studied by comparing the nucleotide sequences of 27 clones obtained from two rhesus monkeys infected with molecularly cloned simian immunodeficiency virus. All 27 clones differed from each other and differed from the input clone in the gp120 (SU) portion of the envelope gene. Nucleotide substitutions were shown to accumulate with time at an average rate of 8.5 per 1,000 per year in SU. Surprisingly, the majority of nucleotide substitutions (81%) resulted in amino acid changes. Variation in SU was not random but occurred predominantly in five discrete regions. Within these variable regions, a remarkable 98% of the nucleotide substitutions changed the amino acid. These results demonstrate that extensive sequence variability accumulates in vivo after infection with molecularly cloned virus and that selection occurs in vivo for changes in distinct variable regions in env.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Viral/biosynthesis Antigenic Variation Base Sequence Cell Line Cloning, Molecular Gene Products, env/genetics,immunology Genotype Immunoglobulin Variable Region/genetics,immunology Macaca mulatta Molecular Sequence Data Retroviridae Proteins, Oncogenic/genetics,immunology Simian Acquired Immunodeficiency Syndrome/immunology Simian Immunodeficiency Virus/genetics,immunology Viral Fusion Proteins
Chemicals
Antibodies, Viral Gene Products, env Immunoglobulin Variable Region Retroviridae Proteins, Oncogenic Viral Fusion Proteins transmembrane protein, Simian immunodeficiency virus
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Burns D P
New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772.
Desrosiers R C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.4.1843-1854.1991
Published
1991-04-00
Pages
1843-54
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC239994
Subset
IM
Grants
NIAID NIH HHS · AI25328 · United States
NIAID NIH HHS · AI26463 · United States
NCRR NIH HHS · RR00168 · United States
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GENBANK
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External Links
PubMed source DOI: 10.1128/JVI.65.4.1843-1854.1991 PMC Full Text View journal details
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