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PMID: 9015303 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized cells.

The Journal of cell biology ·Vol. 136 ·No. 2 ·1997-01-27 ·Pages 319-30

Wilson JM, Colton TL

Abstract

Polarized cells such as epithelial cells and neurons have distinct endosomal compartments associated with different plasma membrane domains. The endosomes of the neuronal cell body and the basolateral cytoplasm of epithelial cells are thought to perform cellular "housekeeping" functions such as the uptake of nutrients and metabolites, while the endosomes in the apical cytoplasm or axons are thought to be specialized for the sorting and transcytosis of cell type-specific ligands and receptors. However, it is not known if nonpolarized cells such as fibroblasts contain a specialized endosomal compartment analogous to the specialized endosomes found in neurons and epithelia. We have expressed a protein that is normally found in the apical early endosomes of developing intestinal epithelial cells in normal rat kidney fibroblasts. This apical endosomal marker, called endotubin, is targeted to early endosomes in transfected fibroblasts, and is present in peripheral as well as perinuclear endosomes. The peripheral endosomes that contain endotubin appear to exclude transferrin, fluid phase markers, and the mannose-6-phosphate receptor, although in the perinuclear region colocalization of endotubin and these markers is present. In addition, endotubin positive structures do not tubulate in response to brefeldin A and instead redistribute to a diffuse perinuclear location. Since this endosomal compartment has many of the characteristics of an apical or axonal endosomal compartment, our results indicate that nonpolarized cells also contain a specialized early endosomal compartment.

MeSH Terms
Animals Animals, Newborn Brefeldin A Cell Compartmentation Cell Line Cell Nucleus/ultrastructure Cell Polarity Clathrin/metabolism Cyclopentanes/pharmacology Endocytosis Endosomes/drug effects,metabolism,ultrastructure Fibroblasts/drug effects,metabolism,ultrastructure Glycoproteins/metabolism Ileum/drug effects,metabolism Intestinal Mucosa/drug effects,metabolism Ovalbumin/metabolism Rats Receptor, IGF Type 2/metabolism Ricin/metabolism Transfection Transferrin/metabolism
Chemicals
Clathrin Cyclopentanes Glycoproteins Receptor, IGF Type 2 Transferrin Brefeldin A Ovalbumin Ricin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson J M
Department of Cell Biology and Anatomy, Steele Memorial Children's Research Center, University of Arizona, Tucson 85724, USA. jwilson@biosci.arizona.edu
Colton T L
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.136.2.319
Email
jwilson@biosci.arizona.edu
Published
1997-01-27
Pages
319-30
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2134826
Subset
IM
Grants
NIDDK NIH HHS · DK43329 · United States
External Links
PubMed source DOI: 10.1083/jcb.136.2.319 PMC Full Text View journal details
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