Home LiteratureArticle Details

PMID: 7515888 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In migrating fibroblasts, recycling receptors are concentrated in narrow tubules in the pericentriolar area, and then routed to the plasma membrane of the leading lamella.

The Journal of cell biology ·Vol. 125 ·No. 6 ·1994-06-00 ·Pages 1265-74

Hopkins CR, Gibson A, Shipman M, Strickland DK, Trowbridge IS

Abstract

By following the intracellular processing of recycling transferrin receptors and the selective sorting of a-2 macroglobulin in chick embryo fibroblasts, we have shown that the concentration of 60 nm diam tubules which surrounds the centrioles represents a distal compartment on the recycling pathway. In migrating cells transferrin receptor tracers can be loaded into this compartment and then chased to the cell surface. When they emerge the recycling transferrin receptors are distributed over the surface of the leading lamella.

MeSH Terms

Animals Biological Transport Cell Compartmentation Cell Membrane/metabolism Cell Movement/physiology Cells, Cultured Centrioles/metabolism Chick Embryo Fibroblasts/physiology Intracellular Membranes/metabolism Lysosomes/physiology Microscopy, Fluorescence Microscopy, Immunoelectron Receptors, Transferrin/isolation & purification,metabolism Transferrin/isolation & purification,metabolism alpha-Macroglobulins/metabolism

Chemicals

Receptors, Transferrin Transferrin alpha-Macroglobulins

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Hopkins C R

Laboratory of Molecular Cell Biology, University College, London, United Kingdom.

Gibson A

Shipman M

Strickland D K

Trowbridge I S

References (26)

26 references, click to expand

Sorting of membrane components from endosomes and subsequent recycling to the cell surface occurs by a bulk flow process.
J Cell Biol. 1993 Jun;121(6):1257-69 PMID: 8509447
Depletion of intracellular potassium disrupts coated pits and reversibly inhibits cell polarization during fibroblast spreading.
J Cell Biol. 1993 Mar;120(6):1449-59 PMID: 8449988
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
J Histochem Cytochem. 1966 Apr;14(4):291-302 PMID: 5962951
Human alpha2-macroglobulin.
Methods Enzymol. 1976;45:639-52 PMID: 64914
Membrane flow during pinocytosis. A stereologic analysis.
J Cell Biol. 1976 Mar;68(3):665-87 PMID: 1030706
Mechanisms of cellular adhesion. IV. Role of serum glycoproteins in fibroblast spreading on glass.
J Cell Sci. 1979 Feb;35:281-305 PMID: 106061
Binding of apotransferrin to K562 cells: explanation of the transferrin cycle.
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2263-6 PMID: 6300904
Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.
J Cell Biol. 1983 Aug;97(2):508-21 PMID: 6309862
Endocytosis: relation to capping and cell locomotion.
Science. 1984 May 18;224(4650):681-6 PMID: 6719108
Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway.
Cell. 1984 Jul;37(3):789-800 PMID: 6204769
The appearance and internalization of transferrin receptors at the margins of spreading human tumor cells.
Cell. 1985 Jan;40(1):199-208 PMID: 2981629
A new method of preparing gold probes for multiple-labeling cytochemistry.
Eur J Cell Biol. 1985 Jul;38(1):87-93 PMID: 4029177
The directed migration of eukaryotic cells.
Annu Rev Cell Biol. 1986;2:337-65 PMID: 3548771
Identification of the intermolecular disulfide bonds of the human transferrin receptor and its lipid-attachment site.
EMBO J. 1987 Feb;6(2):327-31 PMID: 3582362
Translocation and clustering of endosomes and lysosomes depends on microtubules.
J Cell Biol. 1987 Sep;105(3):1253-65 PMID: 3308906
Dynamic behavior of the transferrin receptor followed in living epidermoid carcinoma (A431) cells with nanovid microscopy.
Cell Motil Cytoskeleton. 1988;9(1):30-47 PMID: 2895685
Kinetics of alpha 2-macroglobulin endocytosis and degradation in mutant and wild-type Chinese hamster ovary cells.
J Cell Physiol. 1989 May;139(2):377-82 PMID: 2469687
Nanometre-level analysis demonstrates that lipid flow does not drive membrane glycoprotein movements.
Nature. 1989 Jul 27;340(6231):284-8 PMID: 2747796
Iterative fractionation of recycling receptors from lysosomally destined ligands in an early sorting endosome.
J Cell Biol. 1989 Dec;109(6 Pt 2):3303-14 PMID: 2600137
Role of the human transferrin receptor cytoplasmic domain in endocytosis: localization of a specific signal sequence for internalization.
J Cell Biol. 1990 Feb;110(2):283-94 PMID: 2298808
Microtubule-based motor proteins.
Curr Opin Cell Biol. 1990 Feb;2(1):15-22 PMID: 2139334
Movement of internalized ligand-receptor complexes along a continuous endosomal reticulum.
Nature. 1990 Jul 26;346(6282):335-9 PMID: 2374607
Clathrin, adaptors, and sorting.
Annu Rev Cell Biol. 1990;6:151-71 PMID: 2177341
Tubular early endosomal networks in AtT20 and other cells.
J Cell Biol. 1991 Nov;115(3):635-53 PMID: 1918157
Polarity of epithelial and neuronal cells.
Annu Rev Cell Biol. 1992;8:395-427 PMID: 1476804
Signal-dependent membrane protein trafficking in the endocytic pathway.
Annu Rev Cell Biol. 1993;9:129-61 PMID: 8280459

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.125.6.1265

Published

1994-06-00

Pages

1265-74

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2290921

Subset

External Links

PubMed source DOI: 10.1083/jcb.125.6.1265 PMC Full Text View journal details

Analysis Services

In migrating fibroblasts, recycling receptors are concentrated in narrow tubules in the pericentriolar area, and then routed to the plasma membrane of the leading lamella.

Contact

WeChat Official Account