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PMID: 2478669 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Acidification and endosome-like compartments in the presynaptic terminals of frog retinal photoreceptors.

Journal of neurocytology ·Vol. 18 ·No. 4 ·1989-08-00 ·Pages 529-40

Sulzer D, Holtzman E

Abstract

By using the 'acidotropic' vital dye, Acridine Orange, we have found that the presynaptic terminals of rod and cone photoreceptors in retinas of Rana pipiens maintain a low pH relative to the surrounding medium through an energy dependent mechanism. When this pH is raised, by exposing the retinas to weak bases like ammonium chloride, the terminals exhibit large, membrane-delimited compartments, many of which accumulate endocytic tracers. This effect is partly reversed when the weak bases are removed. We infer that among the acidified structures within the terminals are endocytic compartments with at least some of the characteristics of the endosomes that participate in receptor-mediated endocytosis in other cell types. One role of these structures in the terminals may be in the recycling of synaptic vesicles.

MeSH Terms
Acridine Orange/metabolism Animals Endocytosis Horseradish Peroxidase/metabolism Hydrogen-Ion Concentration Organelles/physiology,ultrastructure Photoreceptor Cells/physiology,ultrastructure Rana pipiens Retina/physiology,ultrastructure Staining and Labeling
Chemicals
Horseradish Peroxidase Acridine Orange
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sulzer D
Department of Biological Sciences, Columbia University, New York 10027.
Holtzman E
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Article Info
Journal
Journal of neurocytology
Abbr.
J Neurocytol
ISSN
0300-4864
DOI
10.1007/BF01474548
Published
1989-08-00
Pages
529-40
Language
English
Region
United States
NLM ID
0364620
PMCID
PMC7089201
Subset
IM
Grants
NEI NIH HHS · EY-03168 · United States
External Links
PubMed source DOI: 10.1007/BF01474548 PMC Full Text
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