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PMID: 2448272 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma cells. A flow-cytometry and cytochemical study.

Histochemistry ·Vol. 87 ·No. 5 ·1987-00-00 ·Pages 445-55

Báguena-Cervellera R, Renau-Piqueras J, O'Connor JE, Grisolia S

Abstract

The effect of prolonged exposure to ammonia on fluid-phase, receptor-mediated, and adsorptive (non specific) endocytosis in cultured neuroblastoma (Neuro-2a) cells were studied using fluorescein-labeled dextran, concanavalin A conjugated with fluorescein isothiocyanate, and cationized ferritin as tracers. Ammonia treatment increased the rate of endocytosis of cationized ferritin as well as the number of cell elements involved in the process. Moreover, the number of cytoplasmic components containing acid phosphatase activity was also found to increase following ammonia treatment. In contrast, flow-cytometric analyses showed that, under experimental conditions, exposure to ammonia did not alter the intralysosomal pH and had little effect on the fluid-phase and receptor-mediated endocytosis of fluorescein-labeled dextran and concanavalin-A fluorocrome, respectively.

MeSH Terms
Acetylcholinesterase/analysis Acid Phosphatase/analysis Ammonia/toxicity Animals Concanavalin A/analogs & derivatives Dextrans Endocytosis/drug effects Ferritins Flow Cytometry Fluorescein-5-isothiocyanate/analogs & derivatives Fluoresceins Histocytochemistry Hydrolyzable Tannins/pharmacology Neuroblastoma Time Factors Tumor Cells, Cultured
Chemicals
Dextrans Fluoresceins Hydrolyzable Tannins fluorescein isothiocyanate-concanavalin A fluorescein-dextran polycationic ferritin Concanavalin A Ammonia Ferritins Acetylcholinesterase Acid Phosphatase Fluorescein-5-isothiocyanate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Báguena-Cervellera R
Instituto de Investigaciones Citologicas, Valencia, Spain.
Renau-Piqueras J
O'Connor J E
Grisolia S
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00496816
Published
1987-00-00
Pages
445-55
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00496816
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