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PMID: 8967907 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators.

Nucleic acids research ·Vol. 24 ·No. 23 ·1996-12-01 ·Pages 4599-607

Schjerling P, Holmberg S

Abstract

The C6 zinc cluster family of fungal regulatory proteins shares as DNA-binding motif the C6 zinc cluster, also known as the Zn(II)2Cys6 binuclear cluster. This family includes transcriptional activators like Gal4p, Leu3p, Hap1p, Put3p and Cha4p from Saccharomyces cerevisiae, qutA and amdR from Aspergillus, nit4 from Neurospora and Ntf1 from Schizosaccharomyces pombe. Seventy-nine proteins were retrieved from databases by homology to the C6 zinc cluster. All were fungal and 56 were found in the entire genome sequence of S.cerevisiae. Sequence analysis suggests that 60 of the 79 proteins possess one or more coiled-coil dimerization regions succeeding the C6 zinc cluster. Previous comparisons of Gal4p and seven other C6 zinc cluster proteins identified an additional region with weak homology. This region, designated the middle homology region (MHR), was shown to be present in 50 of the 79 proteins. Although reported mutation and deletion analyses suggest a role of MHR in regulation of protein activity, no function has yet been assigned specifically to this region. We find that the family of MHR sequences is confined to C6 zinc cluster proteins and hypothesize that one MHR function is to assist the C6 zinc cluster in DNA target discrimination.

MeSH Terms
Amino Acid Sequence Binding Sites DNA/chemistry,metabolism DNA-Binding Proteins Dimerization Fungal Proteins/chemistry Molecular Sequence Data Protein Structure, Secondary Saccharomyces cerevisiae/chemistry Saccharomyces cerevisiae Proteins Sequence Analysis Sequence Homology Transcription Factors/chemistry Zinc
Chemicals
DNA-Binding Proteins Fungal Proteins GAL4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors DNA Zinc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schjerling P
Department of Genetics, Institute of Molecular Biology, University of Copenhagen, Denmark.
Holmberg S
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/24.23.4599
Published
1996-12-01
Pages
4599-607
Language
English
Region
England
NLM ID
0411011
PMCID
PMC146297
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/24.23.4599 PMC Full Text View journal details
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