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PMID: 2046677 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Complex transcriptional regulation of the Saccharomyces cerevisiae CYB2 gene encoding cytochrome b2: CYP1(HAP1) activator binds to the CYB2 upstream activation site UAS1-B2.

Molecular and cellular biology ·Vol. 11 ·No. 7 ·1991-07-00 ·Pages 3762-72

Lodi T, Guiard B

Abstract

Expression of the Saccharomyces cerevisiae gene encoding cytochrome b2 (EC 1.2.2.3), CYB2, was investigated by direct analysis of mRNA transcripts and by measurement of the expression of lacZ fused to the CYB2 control regions. These studies indicated that regulation of the CYB2 gene is subject to several metabolic controls at the transcriptional level: inhibition due to glucose fermentation, induction by lactate, and inhibition in anaerobiosis or in absence of heme biosynthesis. Furthermore, we have shown that the CYB2 promoter contains one cis negative regulatory region and two heme-dependent positive regions, one of which is controlled by the transcriptional regulator CYP1 (HAP1) which is involved in the modulation of the expression of several oxygen-regulated genes. The CYP1 (HAP1)-binding sequence was located by gel retardation and DNase I footprinting experiments and compared with the binding sequences previously characterized in detail (UAS1CYC1, UAS'CYP3 (CYC7), and UASCTT1).

MeSH Terms
Base Sequence Binding Sites Chromosome Deletion DNA, Fungal/genetics,isolation & purification DNA-Binding Proteins/metabolism Fungal Proteins/metabolism Gene Expression Regulation, Fungal Genes, Fungal L-Lactate Dehydrogenase/genetics L-Lactate Dehydrogenase (Cytochrome) Molecular Sequence Data Oligonucleotide Probes Promoter Regions, Genetic RNA, Messenger/genetics,metabolism Restriction Mapping Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Trans-Activators/metabolism Transcription Factors Transcription, Genetic
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins HAP1 protein, S cerevisiae Oligonucleotide Probes RNA, Messenger Saccharomyces cerevisiae Proteins Trans-Activators Transcription Factors L-Lactate Dehydrogenase L-Lactate Dehydrogenase (Cytochrome)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lodi T
Institute of Genetics, University of Parma, Italy.
Guiard B
References (45)
45 references, click to expand
  1. Yeast/E. coli shuttle vectors with multiple unique restriction sites.
    Yeast. 1986 Sep;2(3):163-7 PMID: 3333305
  2. Co-ordinate control of synthesis of mitochondrial and non-mitochondrial hemoproteins: a binding site for the HAP1 (CYP1) protein in the UAS region of the yeast catalase T gene (CTT1).
    EMBO J. 1988 Jun;7(6):1799-804 PMID: 2844525
  3. CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. I. Overall organization of the protein sequence displays several novel structural domains.
    J Mol Biol. 1988 Nov 20;204(2):263-76 PMID: 2851658
  4. CYP1 (HAP1) regulator of oxygen-dependent gene expression in yeast. II. Missense mutation suggests alternative Zn fingers as discriminating agents of gene control.
    J Mol Biol. 1988 Nov 20;204(2):277-82 PMID: 2851659
  5. Mutations that alter transcriptional activation but not DNA binding in the zinc finger of yeast activator HAPI.
    Nature. 1989 Nov 9;342(6246):200-3 PMID: 2509943
  6. Positive and negative transcriptional control by heme of genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase in Saccharomyces cerevisiae.
    Mol Cell Biol. 1989 Dec;9(12):5702-12 PMID: 2685574
  7. Internal deletions in the yeast transcriptional activator HAP1 have opposite effects at two sequence elements.
    Proc Natl Acad Sci U S A. 1990 Jun;87(12):4524-8 PMID: 2162046
  8. A cis-acting element present in multiple genes serves as a repressor protein binding site for the yeast CAR1 gene.
    Mol Cell Biol. 1990 Aug;10(8):3884-95 PMID: 2115115
  9. [MODE OF ACTION OF LACTATE DEHYDROGENASES LINKED TO FLAVIN AND CYTOCHROME SYSTEMS].
    Bull Soc Chim Biol (Paris). 1964;46:1793-828 PMID: 14270552
  10. [INDUCTION OF LACTATE-CYTOCHROME C REDUCTASES (D- AND L-) OF AEROBIC YEAST BY LACTATES (D- AND L-)].
    Biochim Biophys Acta. 1965 Feb 15;97:183-201 PMID: 14294759
  11. Genetics and biosynthesis of cytochrome c.
    Annu Rev Genet. 1971;5:257-96 PMID: 16097658
  12. Functional dissection and sequence of yeast HAP1 activator.
    Cell. 1989 Jan 27;56(2):291-301 PMID: 2643482
  13. Calcium-dependent bacteriophage DNA infection.
    J Mol Biol. 1970 Oct 14;53(1):159-62 PMID: 4922220
  14. Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring delta-aminolevulinic acid.
    J Biol Chem. 1975 Dec 10;250(23):9090-8 PMID: 127792
  15. [Network of interactions between unlinked genes: synergistic and antagonistic regulation of iso-1-cytochrome c, iso-2-cytochrome c and cytochrome b2 synthesis].
    Biochimie. 1976;58(1-2):155-72 PMID: 182283
  16. Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.
    Proc Natl Acad Sci U S A. 1979 Jan;76(1):343-7 PMID: 154672
  17. CYP3--15: an up-promoter mutation at the ISO-2 cytochrome c structural gene locus and its interaction with other independent regulatory mutations of cytochrome c synthesis in Saccharomyces cerevisiae.
    Mol Gen Genet. 1979 Sep;175(2):209-16 PMID: 229386
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  20. Genes affecting the expression of cytochrome c in yeast: genetic mapping and genetic interactions.
    Genetics. 1980 Apr;94(4):871-89 PMID: 6254831
  21. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2199-203 PMID: 6264467
  22. The molecular characterization of three transcriptional mutations in the yeast iso-2-cytochrome c gene.
    J Biol Chem. 1982 Jul 10;257(13):7756-61 PMID: 6282853
  23. Differential accumulation of two apo-iso-cytochromes c in processing mutants of yeast.
    J Biol Chem. 1982 Aug 25;257(16):9811-21 PMID: 6286634
  24. Regulation of synthesis of catalases and iso-1-cytochrome c in Saccharomyces cerevisiae by glucose, oxygen and heme.
    Eur J Biochem. 1982 Nov;128(1):179-84 PMID: 6293826
  25. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  26. Transient kinetics of the one-electron transfer reaction between reduced flavocytochrome b2 and oxidized cytochrome c. Evidence for the existence of a protein complex in the reaction.
    Eur J Biochem. 1982 Nov 15;128(2-3):533-42 PMID: 6295760
  27. A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7410-4 PMID: 6760197
  28. Modulator sequences mediate oxygen regulation of CYC1 and a neighboring gene in yeast.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):151-5 PMID: 6296862
  29. Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site.
    Cell. 1983 Apr;32(4):1279-86 PMID: 6301690
  30. Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.
    Cell. 1984 Feb;36(2):503-11 PMID: 6319028
  31. A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.
    Mol Cell Biol. 1984 Oct;4(10):2023-30 PMID: 6095036
  32. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  33. Regulation of the expression of iso 2-cytochrome c gene in S. cerevisiae: cloning of the positive regulatory gene CYP1 and identification of the region of its target sequence on the structural gene CYP3.
    Mol Gen Genet. 1985;199(3):524-33 PMID: 2993801
  34. Structure, expression and regulation of a nuclear gene encoding a mitochondrial protein: the yeast L(+)-lactate cytochrome c oxidoreductase (cytochrome b2).
    EMBO J. 1985 Dec 1;4(12):3265-72 PMID: 3004948
  35. Heme control region of the catalase T gene of the yeast Saccharomyces cerevisiae.
    Mol Gen Genet. 1986 Apr;203(1):73-8 PMID: 2423850
  36. A single Saccharomyces cerevisiae upstream activation site (UAS1) has two distinct regions essential for its activity.
    Mol Cell Biol. 1986 Dec;6(12):4690-6 PMID: 3025665
  37. Yeast HAP1 activator binds to two upstream activation sites of different sequence.
    Cell. 1987 Apr 10;49(1):19-27 PMID: 3030565
  38. Ubiquitous upstream repression sequences control activation of the inducible arginase gene in yeast.
    Proc Natl Acad Sci U S A. 1987 Jun;84(12):3997-4001 PMID: 3295874
  39. Regulation of sugar utilization in Saccharomyces species.
    J Bacteriol. 1987 Nov;169(11):4873-7 PMID: 3312161
  40. Organization of the regulatory region of the yeast CYC7 gene: multiple factors are involved in regulation.
    Mol Cell Biol. 1987 Sep;7(9):3252-9 PMID: 2823123
  41. Successive translocation into and out of the mitochondrial matrix: targeting of proteins to the intermembrane space by a bipartite signal peptide.
    Cell. 1987 Dec 24;51(6):1027-37 PMID: 2826012
  42. The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c.
    Curr Genet. 1986;10(5):339-42 PMID: 2832070
  43. A liver-specific DNA-binding protein recognizes multiple nucleotide sites in regulatory regions of transthyretin, alpha 1-antitrypsin, albumin, and simian virus 40 genes.
    Proc Natl Acad Sci U S A. 1988 Jun;85(11):3840-4 PMID: 2836860
  44. Oxygen-dependent upstream activation sites of Saccharomyces cerevisiae cytochrome c genes are related forms of the same sequence.
    Mol Cell Biol. 1988 Jun;8(6):2275-9 PMID: 2841577
  45. Gene regulation by steroid hormones.
    Cell. 1989 Feb 10;56(3):335-44 PMID: 2644044
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.7.3762-3772.1991
Published
1991-07-00
Pages
3762-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361146
Subset
IM
Databases
GENBANK
X03215
External Links
PubMed source DOI: 10.1128/mcb.11.7.3762-3772.1991 PMC Full Text View journal details
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