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PMID: 7957085 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A zinc finger protein, essential for chromosome segregation, constitutes a putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore complex, Cbf3.

The EMBO journal ·Vol. 13 ·No. 21 ·1994-11-01 ·Pages 5203-11

Lechner J

Abstract

A multisubunit protein complex, Cbf3, is a component of the Saccharomyces cerevisiae kinetochore. Cbf3 was recently shown to be essential for chromosome segregation in vivo and for movement of centromere DNA (CEN) along microtubules in vitro. Cbf3 contains three proteins, Cbf3a, Cbf3b and Cbf3c. Here the characterization of Cbf3b is described. Cbf3b contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain and a putative coiled coil dimerization domain. Cbf3b is essential for growth. Mutations within the zinc finger domain result in cells that exhibit a G2-M cell cycle delay and increased chromosome loss in each mitotic cell division. Therefore, Cbf3b has an essential function in chromosome segregation and the zinc finger domain executes part of this function presumably by providing the specific interaction between Cbf3 and CEN. Finally, data are provided to show that Cbf3c is encoded by CTF13, a gene that had been cloned recently by complementing a temperature sensitive mutant that exhibits chromosome loss as a result of a defective centromere.

Related Genes
CTF13
MeSH Terms
Amino Acid Sequence Base Sequence Chromosomes/physiology Cloning, Molecular DNA-Binding Proteins/genetics,metabolism Escherichia coli/genetics Fungal Proteins/genetics,metabolism Genes, Fungal/genetics Kinetochores/chemistry Mitosis Molecular Sequence Data Mutation Nuclear Proteins/genetics,metabolism Peptides/isolation & purification Phenotype Protein Conformation Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Analysis Zinc Fingers
Chemicals
CEP3 protein, S cerevisiae CTF13 protein, S cerevisiae DNA-Binding Proteins Fungal Proteins Nuclear Proteins Peptides Recombinant Proteins Saccharomyces cerevisiae Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Lechner J
Institut für Biochemie, Genetik und Mikrobiologie, Universität Regensburg, Germany.
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-11-01
Pages
5203-11
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395469
Subset
IM
Databases
GENBANK
X81396
External Links
PubMed source PMC Full Text View journal details
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